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Determination of protein, fat, starch, and amino acids in foxtail millet [Setaria italica (L.) Beauv.] by Fourier transform near-infrared reflectance spectroscopy

机译:傅里叶变换近红外反射光谱法测定谷子[Setaria italica(L.)Beauv。]中的蛋白质,脂肪,淀粉和氨基酸

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摘要

Quantitative detection of protein, fat, starch, and amino acids in foxtail millet using Fourier transform near-infrared spectroscopy (NIRS) was investigated. Foxtail millet samples (n=259) were analyzed using NIRS. Spectral data were linearized with data from chemical analyses. Calibration models were established using a partial least-squares (PLS) algorithm with cross-validation. Optimized models were tested using external validation set samples with coefficients of determination in the external validation (R 2 val) of >0.90. Residual predictive deviation (RPD) values were nearly equal to or >2.5 for crude protein, alanine, aspartic acid, glutamic acid, isoleucine, leucine, and serine. However, for glycine, histidine, phenylalanine, proline, threonine, tyrosine, and valine, the R 2 val values were >0.83 and RPD values were nearly equal to or >2.0. For crude fat, total starch, arginine, and lysine, the R 2 val values were >0.70 and RPD values were >1.5. NIRS is a rapid determination tool for foxtail millet breeding, and for quality control.
机译:研究了使用傅立叶变换近红外光谱(NIRS)定量检测谷子中蛋白质,脂肪,淀粉和氨基酸的方法。使用NIRS分析了谷子样品(n = 259)。光谱数据与化学分析数据线性化。使用带有交叉验证的偏最小二乘(PLS)算法建立校准模型。使用外部验证集样本测试优化的模型,外部验证的确定系数(R 2 val)> 0.90。粗蛋白,丙氨酸,天冬氨酸,谷氨酸,异亮氨酸,亮氨酸和丝氨酸的残留预测偏差(RPD)值几乎等于或大于2.5。但是,对于甘氨酸,组氨酸,苯丙氨酸,脯氨酸,苏氨酸,酪氨酸和缬氨酸,R 2 val值> 0.83,RPD值几乎等于或> 2.0。对于粗脂肪,总淀粉,精氨酸和赖氨酸,R 2 val值> 0.70,RPD值> 1.5。 NIRS是用于谷子育种和质量控制的快速测定工具。

著录项

  • 来源
    《Food Science and Biotechnology》 |2013年第6期|1495-1500|共6页
  • 作者单位

    Research Center of Environmental Science and Engineering Shanxi University">(1);

    Institute of Crop Science Chinese Academy of Agricultural Sciences">(2);

    Institute of Crop Science Chinese Academy of Agricultural Sciences">(2);

    School of Chemistry and Chemical Engineering Shanxi University">(3);

    Institute of Crop Science Chinese Academy of Agricultural Sciences">(2);

    School of Chemistry and Chemical Engineering Shanxi University">(3);

    Institute of Crop Science Chinese Academy of Agricultural Sciences">(2);

    Institute of Crop Science Chinese Academy of Agricultural Sciences">(2);

    Institute of Crop Science Chinese Academy of Agricultural Sciences">(2);

    Institute of Crop Science Chinese Academy of Agricultural Sciences">(2);

    Institute of Crop Science Chinese Academy of Agricultural Sciences">(2);

    Institute of Crop Science Chinese Academy of Agricultural Sciences">(2);

    Institute of Crop Science Chinese Academy of Agricultural Sciences">(2);

    Research Center of Environmental Science and Engineering Shanxi University">(1);

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    foxtail millet; protein; starch; amino acid; NIRS;

    机译:谷子蛋白;淀粉;氨基酸;近红外;

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