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Isolation and identification of antioxidative compounds and their activities from Suaeda japonica

机译：Su木中抗氧化化合物的分离鉴定及其活性

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摘要

The ethyl acetate (EtOAc) and chloroform (CHCl3) layers obtained after solvent fractionation of a H2O suspension of powdered Suaeda japonica juice showed higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicalscavenging activity than other layers. Eighteen compounds were purified and isolated from the EtOAc and CHCl3 layers using chromatography following DPPH radicalscavenging assay. These compounds were identified as dihydroferulic acid methyl ester (1), pyrocatechol (2), syringic acid (3), apigenin (4), isorhamnetin (5), kaempferol (6), dihydroferulic acid (7), vanillic acid (8), 4-hydroxybenzoic acid (9), acetophlorglucine (10), homoeriodictyol (11), naringenin (12), quercetin (13), luteolin (14), 9-epiblumenol C (15), scopoletin (16), dihydroisorhamnetin (17), and chrysoeriol (18). The structures of these compounds were determined by mass spectrometry and nuclear magnetic resonance analyses. The isolated compounds were newly identified from this plant. Compounds 13 and 14 exhibited higher DPPH radical-scavenging activity and an inhibition effect against ferric ion-induced lipid oxidation of rat liver when compared to α-tocopherol and other compounds.
机译：粉末状Suedae粳汁的H2O悬浮液的溶剂分馏后获得的乙酸乙酯（EtOAc）和氯仿（CHCl3）层显示出比其他层更高的1,1-二苯基-2-吡啶并肼基（DPPH）自由基清除活性。在DPPH自由基清除测定之后，使用色谱法纯化并从EtOAc和CHCl 3层中分离出十八种化合物。这些化合物被鉴定为二氢阿魏酸甲酯（1），邻苯二酚（2），丁香酸（3），芹菜素（4），异鼠李素（5），山emp酚（6），二氢阿魏酸（7），香草酸（8） ，4-羟基苯甲酸（9），乙酰苯芦碱（10），高柔顺丁烯醇（11），柚皮苷（12），槲皮素（13），木犀草素（14），9-表布三烯醇C（15），大麦草素（16），二氢异山梨糖素（17） ）和金鸡油（18）。这些化合物的结构通过质谱和核磁共振分析确定。从该植物中新鉴定出分离的化合物。与α-生育酚和其他化合物相比，化合物13和14表现出更高的DPPH自由基清除活性和对铁离子诱导的大鼠肝脏脂质氧化的抑制作用。

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  • 来源
    《Food Science and Biotechnology》 |2013年第6期|1547-1557|共11页
  • 作者

    Jeong-Yong Cho; Xing Yang; Kyung-Hee Park; Hye Jin Park; Sun-Young Park; Jae-Hak Moon; Kyung-Sik Ham;

  • 作者单位

    Department of Food Science Biotechnology and Solar Salt Biotechnology Research Center Mokpo National University">(1);

    Department of Food Science Biotechnology and Solar Salt Biotechnology Research Center Mokpo National University">(1);

    Department of Food Science Biotechnology and Solar Salt Biotechnology Research Center Mokpo National University">(1);

    Department of Food Science Biotechnology and Solar Salt Biotechnology Research Center Mokpo National University">(1);

    Department of Food Science Biotechnology and Solar Salt Biotechnology Research Center Mokpo National University">(1);

    Department of Food Science Technology and Functional Food Research Center Chonnam National University">(2);

    Department of Food Science Biotechnology and Solar Salt Biotechnology Research Center Mokpo National University">(1);

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  • 正文语种 eng
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  • 关键词

    Suaeda japonica; antioxidant; flavonoid; phenolics; DPPH radical-scavenging activity;

    机译：Suaeda japonica;抗氧化剂类黄酮酚醛树脂DPPH自由基清除活性;

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