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Extraction of carotenoids from Phaffia rhodozyma: A comparison between different techniques of cell disruption

机译：红发夫酵母中类胡萝卜素的提取：不同细胞破碎技术之间的比较

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摘要

The yeast Phaffia rhodozyma is known for producing carotenogenic pigments, commonly used in aquaculture feed formulation as well as in cosmetic, pharmaceutical, and food industries. Despite the high production of carotenoids from microorganisms by biotechnology, their use has limitation due to the cell wall resistance, which constitutes a barrier to the bioavailability of carotenoids. Therefore, there is a need to improve carotenoids recovering technique from microorganisms for the application of food industries. This study aimed to compare mechanical, chemical, and enzymatic techniques of cell disruption for extracting carotenoids produced by P. rhodozyma NRRL Y-17268. Among the techniques studied, the highest specific concentration of carotenoids (190.35 μg/g) resulted from the combined techniques of frozen biomass maceration using diatomaceous earth and enzymatic lysis at pH of the reaction medium of 4.5 at 55°C, with initial activity of β-1,3 glucanase of 0.6 U/mL for 30 min.
机译：酵母红发夫酵母（Raffia rhodozyma）已知用于生产致血管生成色素，通常用于水产养殖饲料配方以及化妆品，制药和食品工业中。尽管通过生物技术从微生物中大量生产类胡萝卜素，但由于细胞壁抗性，其使用受到限制，这构成了对类胡萝卜素生物利用度的障碍。因此，需要改进从微生物中回收类胡萝卜素的技术以用于食品工业。这项研究旨在比较细胞分裂的机械，化学和酶促技术，以提取由红假单胞菌NRRL Y-17268产生的类胡萝卜素。在所研究的技术中，类胡萝卜素的最高比浓浓度（190.35μg/ g）来自于使用硅藻土冷冻生物质浸渍和在55°C的反应介质的pH值为4.5的酶解下的酶解的联合技术，β的初始活性为β。 -1,3葡聚糖酶0.6 U / mL，持续30分钟。

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  • 来源
    《Food Science and Biotechnology》 |2012年第1期|p.1-8|共8页
  • 作者

    Mariano Michelon; Thais de Matos de Borba; Ruan da Silva Rafael; Carlos André Veiga Burkert; Janaína Fernandes de Medeiros Burkert;

  • 作者单位

    Bioprocess Engineering Laboratory, School of Chemical and Food, Federal University of Rio Grande, Rua Alfredo Huch 475, 96201-900, Rio Grande, RS, Brazil;

    Bioprocess Engineering Laboratory, School of Chemical and Food, Federal University of Rio Grande, Rua Alfredo Huch 475, 96201-900, Rio Grande, RS, Brazil;

    Bioprocess Engineering Laboratory, School of Chemical and Food, Federal University of Rio Grande, Rua Alfredo Huch 475, 96201-900, Rio Grande, RS, Brazil;

    Bioprocess Engineering Laboratory, School of Chemical and Food, Federal University of Rio Grande, Rua Alfredo Huch 475, 96201-900, Rio Grande, RS, Brazil;

    Bioprocess Engineering Laboratory, School of Chemical and Food, Federal University of Rio Grande, Rua Alfredo Huch 475, 96201-900, Rio Grande, RS, Brazil;

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  • 正文语种 eng
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  • 关键词

    β-1,3 glucanase; enzymatic lysis; dimethyl sulfoxide; biomass; diatomaceous earth;

    机译：β-1,3葡聚糖酶;酶裂解;二甲亚砜;生物质;硅藻土;

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