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首页> 外文期刊>Food Science and Biotechnology >Resistance of Bacillus cereus and its enterotoxin genes in ready-to-eat foods to γ-irradiation
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Resistance of Bacillus cereus and its enterotoxin genes in ready-to-eat foods to γ-irradiation

机译：即食食品中蜡状芽孢杆菌及其肠毒素基因对γ射线的抗性

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摘要

This study was investigated the resistance of Bacillus cereus ATCC 13752 and its enterotoxin coding genes in cereals, vegetables, and tryptic soy broth to γ-irradiation. Screening for enterotoxin coding genes, viability test, PCR analysis, measurement of DNA concentration, cellular constituents release, SEM, and antibiotic sensitivity tests were performed after irradiation at 0, 1.5, 3, 5, 7, 10, and 15 kGy. Thirty % of strains screened possessed all enterotoxins genes. At 10 kGy B. cereus viable cell count was reduced by 4 logCFU/mL. B. cereus in vegetables and broth demonstrated higher susceptibility than those in cereals. Enterotoxins coding genes could be identified in the γ-irradiated cells, antibiotic sensitivity profile was not affected. Concentration of DNA containing enterotoxin coding genes was reduced and release of cellular constituent was highest at 15 kGy. γ-Irradiation up to 10 kGy did not eliminate B. cereus neither affected their enterotoxins coding genes from ready-to-eat food.
机译：本研究调查了谷类，蔬菜和胰蛋白酶大豆肉汤中蜡状芽孢杆菌ATCC 13752及其肠毒素编码基因对γ射线的抗性。在0、1.5、3、5、7、10和15 kGy照射后，进行肠毒素编码基因的筛选，活性测试，PCR分析，DNA浓度测量，细胞成分释放，SEM和抗生素敏感性测试。筛选的菌株中有30％具有所有肠毒素基因。在10 kGy时，蜡状芽孢杆菌的活细胞计数降低了4 logCFU / mL。蔬菜和肉汤中的蜡状芽孢杆菌比谷物中的蜡状芽孢杆菌具有更高的敏感性。可以在经γ射线照射的细胞中鉴定出肠毒素编码基因，抗生素敏感性谱不受影响。含有肠毒素编码基因的DNA浓度降低，细胞成分的释放最高，为15 kGy。高达10 kGy的γ辐照不能消除蜡状芽孢杆菌，也不会影响即食食品中肠毒素编码基因。

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