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首页> 外文期刊>Food Science and Biotechnology >Effect of homogenization speed and time on the recovery of alkaline phosphatase from the hepatopancreatic tissues of shrimps
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Effect of homogenization speed and time on the recovery of alkaline phosphatase from the hepatopancreatic tissues of shrimps

机译：均质化速度和时间对虾肝胰脏组织中碱性磷酸酶恢复的影响

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摘要

Hepatopancreatic tissues of shrimps were homogenized at various pestle speed and time intervals at a constant jerk of 15 passes/min on a narrow space using 0.1 M Tris-HCl buffer of pH 8.4 at 4°C. Release of proteins and alkaline phosphatase were determined by estimating the quantity of protein and alkaline phosphatase activity in each homogenate at the end of the experiments. Optimum condition for the homogenization of the hepatopancreatic cells was achieved at operating speeds of 3,000 rpm for 10 min in the homogenizer. Increase in pestle speed beyond 3,000 rpm for 10 min have no effect on the release of protein. Increasing the disruption speed above 3,000 rpm decreases the specific activity of alkaline phosphatase release, while extending the time of homogenization beyond 10 min at 3,000 rpm or the speed of pestle beyond 3,000 rpm for 10 min caused shear damage to the enzyme.
机译：使用0.1 M Tris-HCl缓冲液（pH 8.4）在4°C下，在狭窄的空间内以15次/分钟的恒定加速度以不同的杵速度和时间间隔匀浆虾的肝胰组织。通过估计实验结束时每个匀浆中蛋白质和碱性磷酸酶活性的量来确定蛋白质和碱性磷酸酶的释放。在均化器中以3,000 rpm的操作速度运行10分钟，达到了肝胰脏细胞均质化的最佳条件。超过3,000 rpm的杵速度增加10分钟对蛋白质的释放没有影响。将破坏速度提高到3,000 rpm以上会降低碱性磷酸酶释放的比活性，同时将均质化时间延长到3,000 rpm时超过10分钟，或者将杵的速度超过3,000 rpm持续10分钟会导致酶的剪切损伤。

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