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Wheat bran globulins: Competitive inhibitors of mushroom tyrosinase

机译:麦麸球蛋白:蘑菇酪氨酸酶的竞争性抑制剂

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摘要

The inhibitory capacity of the globulin fraction of wheat bran against the diphenolase activity of mushroom tyrosinase, using l-tyrosine as substrate, was evaluated. Enzyme kinetics was monitored in the presence of globulin solutions by measuring the absorbance at 475nm. Lineweaver-Burk plots were drawn in order to determine Vmax, Km, and type of inhibition. Results showed that globulins from wheat bran competitively inhibited, the activity of mushroom tyrosinase with a KI of 0.79%(w/v). The degree of inhibition was 24% at 2 mM of the substrate L-tyrosine.
机译:以L-酪氨酸为底物,评价了麦麸球蛋白级分对蘑菇酪氨酸酶双酚酶活性的抑制能力。在球蛋白溶液存在下,通过测量在475nm处的吸光度来监测酶动力学。绘制Lineweaver-Burk图,以确定Vmax ,Km 和抑制类型。结果表明,麦麸中的球蛋白竞争性抑制,蘑菇酪氨酸酶的活性为KI 为0.79%(w / v)。在2 mM的底物L-酪氨酸处,抑制程度为24%。

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