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首页> 外文期刊>Food Science and Biotechnology >Structural characteristics, fluorescence quenching, and antioxidant activity of the arabinogalactan protein-rich fraction from senna (Cassia angustifolia) leaves
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Structural characteristics, fluorescence quenching, and antioxidant activity of the arabinogalactan protein-rich fraction from senna (Cassia angustifolia) leaves

机译:番泻叶(Cassia angustifolia)富含阿拉伯半乳聚糖蛋白的级分的结构特征,荧光猝灭和抗氧化活性

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摘要

Senna (Cassia angustifolia) is widely used in Indian folk medicine for the prevention of various disorders. Herein, we have demonstrated that water extracted arabinogalactan protein (AGP) rich fraction possesses strong antioxidative activity. The AGP rich fraction was analyzed using chemical, chromatographic, and spectroscopic methods. Effect of water extracted polymers on bovine serum albumin spectrum was determined using excitation wavelength of 282 nm. The antioxidant capacity of this fraction was studied by ferric reducing antioxidant power (FRAP) and DPPH radical assays. This polymeric fraction, ≥88% of which gets precipitated with Yariv reagent, consisted mainly of (1→5)-/(1→3,5)-linked α-arabinosyl, (1→3)-/(1→3,6)-linked-galactosyl residues and terminal arabinofuranosyl residues. Its in vitro antioxidant capacity is comparable to that of standard antioxidants. Fluorescence quenching studies furnishes evidence for the interaction of the arabinogalactan protein with bovine serum albumin.
机译:番泻叶(Cassia angustifolia)在印度民间医学中广泛用于预防各种疾病。在此,我们证明了水提取的阿拉伯半乳聚糖蛋白(AGP)富集部分具有很强的抗氧化活性。使用化学,色谱和光谱方法分析富含AGP的馏分。使用282 nm的激发波长确定了水提取的聚合物对牛血清白蛋白光谱的影响。通过三价铁还原抗氧化剂功率(FRAP)和DPPH自由基测定法研究了该级分的抗氧化剂容量。该聚合级分中,≥88%被Yariv试剂沉淀,主要由(1→5)-/(1→3,5)连接的α-阿拉伯糖基,(1→3)-/(1→3, 6)-连接的半乳​​糖基残基和末端阿拉伯呋喃糖基残基。其体外抗氧化能力可与标准抗氧化剂相媲美。荧光猝灭研究为阿拉伯半乳聚糖蛋白与牛血清白蛋白的相互作用提供了证据。

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