首页> 外文期刊>Food science and technology research >In Vitro Antioxidant Activity and In Vivo Anti-fatigue Effects of Oyster (Ostrea plicatula Gmelin) Peptides Prepared Using Neutral Proteinase
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In Vitro Antioxidant Activity and In Vivo Anti-fatigue Effects of Oyster (Ostrea plicatula Gmelin) Peptides Prepared Using Neutral Proteinase

机译:使用中性蛋白酶制备的牡蛎(Ostrea plicatula Gmelin)肽的体外抗氧化活性和体内抗疲劳作用

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摘要

The amino acid profile of oyster (Ostrea plicatula Gmelin) peptides (OP) was assayed. Its in vitro antioxidant activity was determined, and its in vivo anti-fatigue activity was compared with those of oyster meat and oyster protein. Seven amino acids that may play an important role in enhancing antioxidant activity account for 25.8% of total OP content. These amino acids are histidine, proline, methionine, cys-teine, tyrosine, tryptophan, and phenylalanine. The F-value of OP was 4.89, and the concentration of tau-rine was 45.43 mmol/mL. OP acted as a scavenger for hydroxyl radicals, 1,1-diphenyl-2-picrylhydrazyl, and superoxide anion radicals. It also inhibited lipid peroxidation. The intragastric administration of OP prolonged the swimming time to exhaustion of mice by 78% over that of the control. It decreased the levels of serum lactic acid and blood urea nitrogen by 24.8% and 11.2%, respectively. Finally, it increased the levels of liver glycogen (1.0-fold) and gastrocnemius muscle glycogen (55.6%).
机译:测定了牡蛎(Ostrea plicatula Gmelin)肽(OP)的氨基酸谱。测定其体外抗氧化活性,并将其体内抗疲劳活性与牡蛎肉和牡蛎蛋白进行比较。可能在增强抗氧化活性中起重要作用的七个氨基酸占总OP含量的25.8%。这些氨基酸是组氨酸,脯氨酸,蛋氨酸,半胱氨酸,酪氨酸,色氨酸和苯丙氨酸。 OP的F值是4.89,牛磺酸的浓度是45.43mmol / mL。 OP充当羟基自由基,1,1-二苯基-2-甲基苯并肼和超氧阴离子自由基的清除剂。它还抑制脂质过氧化。与对照组相比,胃内给予OP可延长小鼠的疲劳游泳时间78%。它分别使血清乳酸和血尿素氮水平降低了24.8%和11.2%。最后,它增加了肝糖原(1.0倍)和腓肠肌肌肉糖原(55.6%)的水平。

著录项

  • 来源
    《Food science and technology research》 |2013年第4期|623-631|共9页
  • 作者单位

    South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China ,Graduate School of the Chinese Academy of Sciences, Beijing 100049, China ,College of Biological Engineering, Jimei University, Xiamen 361021, China;

    College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China ,Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Zhanjiang 524088, China;

    College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China ,Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Zhanjiang 524088, China;

    College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China ,Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Zhanjiang 524088, China;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    oyster peptides; antioxidant activity; anti-fatigue effect; endurance exercise;

    机译:牡蛎肽抗氧化活性抗疲劳作用;耐力运动;

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