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首页> 外文期刊>Food science and technology research >Purification and Characterization of Polygalacturonase from Screened Aspergillus tubingensis for Coffee Processing
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Purification and Characterization of Polygalacturonase from Screened Aspergillus tubingensis for Coffee Processing

机译：筛选出的曲霉曲霉中多半乳糖醛酸酶的纯化和表征

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摘要

The screened strain that exhibited the highest level of polygalacturonase (PGase) activity based on coffee pectin was selected and identified as Aspergillus tubingensis. Sucrose (2%) and sodium nitrate (3%) were the best carbon and nitrogen sources, respectively, for the strain's production of PGase. At 35℃ and an initial pH of 4 as culture conditions, the strain showed the highest level of PGase production (15.9 U/mL). The highest activity of purified PGase-1 was observed at 60℃ and pH 5. PGase-I activity was highly enhanced by Ca~(2+), Mn~(2+), and Ba~(2+). PGase-I was thermally stable at 50℃, with a half-life of 4 h. High stability was observed after 24 h incubation at pH levels from 3 to 7. The elimination of coffee mucilage was obviously improved by treating with this enzyme.
机译：选择基于咖啡果胶表现出最高水平的聚半乳糖醛酸酶（PGase）活性的菌株，并将其鉴定为管曲霉。蔗糖（2％）和硝酸钠（3％）分别是菌株生产PGase的最佳碳源和氮源。在35℃和初始pH为4的培养条件下，该菌株表现出最高的PGase产生水平（15.9 U / mL）。纯化的PGase-1的最高活性在60℃和pH 5下观察到。Ca〜（2 +），Mn〜（2+）和Ba〜（2+）可大大提高PGase-1的活性。 PGase-I在50℃下具有热稳定性，半衰期为4小时。在pH值为3到7的条件下孵育24小时后，观察到高稳定性。用该酶处理可明显改善咖啡粘液的消除。

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  • 来源
    《Food science and technology research》 |2013年第5期|813-818|共6页
  • 作者

    En-Sheng Tai; Pao-Chuan Hsieh; Shyang-Chwen Sheu;

  • 作者单位

    Department of Food Science, National Pingtung University of Science and Technology, 1 Hseuhfu Road, Neipu, Pingtung 91201, Taiwan;

    Department of Food Science, National Pingtung University of Science and Technology, 1 Hseuhfu Road, Neipu, Pingtung 91201, Taiwan;

    Department of Food Science, National Pingtung University of Science and Technology, 1 Hseuhfu Road, Neipu, Pingtung 91201, Taiwan;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    polygalacturonase; pectin; coffee; Aspergillus tubingensis;

    机译：聚半乳糖醛酸酶;果胶咖啡;油曲霉;

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