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首页> 外文期刊>Frontiers of environmental science & eng >Using mRNA to investigate the effect of low-pressure ultraviolet disinfection on the viability of E. coli
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Using mRNA to investigate the effect of low-pressure ultraviolet disinfection on the viability of E. coli

机译：使用mRNA研究低压紫外线消毒对大肠杆菌生存力的影响

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The efficacy of ultraviolet (UV) disinfection has been analyzed and validated by numerous studies using culture-based methods, yet the discovery of the viable but nonculturable state necessitates the investigation of UV disinfection based on viability parameters. Paired regulators of the SOS response system, recA-lexA, and the programmed cell death system, mazEF, in Escherichia coli were chosen as the target genes, and the effect of UV irradiation on the mRNAs of the four genes was studied. This research showed that, after UV irradiation, the responses of the mRNAs were highly consistent, with reduction percentages of approximately 60% at 20 mJ/cm(2), 70% at 40 mJ/cm(2), and 90% at 80 mJ/cm(2), and these reductions were believed to be the result of direct UV damage to nucleic acids. After 24 h of dark incubation, recA and lexA were both upregulated but to a lesser extent for repressor lexA; and mazE and mazF were both downregulated. This result implies that UV irradiation induces the dark repair system more actively, and the cells will proceed to death at a rate similar to that associated with natural decay.
机译：紫外线（UV）消毒的功效已通过众多基于培养的方法的研究进行了分析和验证，然而，发现可行但不可培养的状态需要根据生存力参数进行紫外线消毒的研究。选择大肠杆菌中SOS响应系统的配对调节子recA-lexA和程序性细胞死亡系统mazEF作为靶基因，并研究了紫外线照射对这四个基因mRNA的影响。这项研究表明，紫外线照射后，mRNA的响应高度一致，在20 mJ / cm（2）时约60％，在40 mJ / cm（2）时约70％，在80时约90％。 mJ / cm（2），这些减少被认为是直接紫外线对核酸造成的损害。在黑暗中孵育24小时后，recA和lexA均被上调，但抑制子lexA的表达程度有所降低。 mazE和mazF均下调。该结果表明，紫外线辐射可更积极地诱导暗处修复系统，并且细胞将以与自然衰变相关的速率相似的速率死亡。

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