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Liquid-liquid extraction: A promising alternative for inhibitors removing of pentoses fermentation

机译：液液萃取：抑制剂去除戊糖发酵的有前途的替代方法

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Dilute sulfuric acid pretreatment is effective at producing a xylose-rich hydrolysate from lignocellulosic biomasses, however inhibitory compounds, released during the processes, can affect the metabolism of fermenting microorganisms, thus reducing the biomass formation, yields and productivity. In this scenario, two approaches for hemicellulosic hydrolysate detoxification were evaluated: liquid-liquid extraction (process 1) and evaporation followed by liquid-liquid extraction (process 2), using 1-butanol, isobutyl acetate and MIBK as extractants. The hydrolysates (detoxified and non-detoxified) fermentations were evaluated by S. stipitis and S. passalidarum. Although process 1 has provided good removal efficiencies, acetic acid concentration in detoxified hydrolysate was still relatively high, while process 2 resulted in 90% of global acetic acid removal. S. passalidarum detoxified hydrolysates fermentation showed higher ethanol yields and productivities than S. stipitis. An inhibitory effect of 1-butanol has been achieved for both yeasts, resulting in low sugar consumption and consequently low ethanoltiters. Process 2 with MIBK led to 69.0% of phenolics; 85.4% of acetic acid and 100.0% of HMF and furfural extractions, with ethanol yield and productivity of 75.6% and 0.41 g/g, respectively. This process comes up as a promising method once it provides toxic-compounds removal without carbohydrates losses and allows an extractant-recycling integrated process.
机译：稀硫酸预处理可有效地从木质纤维素生物质生产富含木糖的水解产物，但是在此过程中释放的抑制性化合物会影响发酵微生物的代谢，从而降低生物质的形成，产量和生产率。在这种情况下，评估了半纤维素水解产物解毒的两种方法：液-液萃取（过程1）和蒸发，然后液-液萃取（过程2），使用1-丁醇，乙酸异丁酯和MIBK作为萃取剂。通过S.stipitis和S.passalidarum对水解产物（解毒和非解毒）发酵进行评估。尽管方法1提供了良好的去除效率，但解毒水解产物中的乙酸浓度仍然相对较高，而方法2则去除了全球90％的乙酸。帕萨里链霉菌解毒的水解产物发酵显示出比S.stipitis更高的乙醇产量和生产力。对两种酵母均已获得了1-丁醇的抑制作用，从而导致了低糖消耗并因此降低了乙醇滴定度。使用MIBK的方法2产生69.0％的酚类; 88.4％的乙酸和100.0％的HMF和糠醛萃取，乙醇收率和产率分别为75.6％和0.41 g / g。该方法一旦提供了有毒化合物的去除而没有碳水化合物的损失，并允许了萃取剂-再循环的集成过程，就成为一种很有前途的方法。

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