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首页> 外文期刊>Genes & Genomics >De novo transcriptome analysis of an Arctic microalga, Chlamydomonas sp.
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De novo transcriptome analysis of an Arctic microalga, Chlamydomonas sp.

机译：从头转录组分析的北极微藻，衣藻。

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摘要

Microalgal Chlamydomonas species are abundant in polar regions and provide a novel model system for investigating the physiological characteristics and mechanisms related to multiple stresses. As part of an effort to understand the adaptation mechanism of polar organisms, we analyzed the transcriptome of an arctic microalgal strain, ArF0006, from the KOPRI Culture Collection of Polar Microorganisms (KCCPM). ArF0006 was isolated from ice-covered freshwater from the Arctic and, therefore, showed optimal growth at low temperatures. It was identified as a Chlamydomonas sp. based on morphology; this was confirmed by molecular phylogenetic analysis. A total of 64,712 reads were generated from pyrosequencing and assembled into 45,443 contigs and 19,424 singletons. Contigs ranged from 100–4,673 bp, with an average of 665 bp. Functional annotations of contigs with a high number of reads were related principally to cold response or photosynthesis. Also, several fatty acid and TGA biosynthesis-related genes and putative homologs of antifreeze protein were identified. A comparison of ArF0006 with Chlamydomonas reinhardtii, Ostreococcus tauri, and Arabidopsis thaliana was performed, and RT-PCR analysis verified the effect of growth temperature on gene expression pattern. Therefore, ArF0006 transcriptome analysis provided useful genetic information regarding cold tolerance that could be useful for bioindustrial applications.
机译：微藻衣藻物种在极地地区丰富，并提供了一种新颖的模型系统来研究与多种胁迫有关的生理特征和机制。为了了解极性生物的适应机制，我们分析了来自北极微生物的KOPRI培养物保藏中心（KCCPM）的北极微藻菌株ArF0006的转录组。 ArF0006是从北极冰层覆盖的淡水中分离出来的，因此在低温下表现出最佳的生长。它被鉴定为衣藻。根据形态分子系统发育分析证实了这一点。焦磷酸测序共产生64,712个读段，并组装成45,443个重叠群和19,424个单例。重叠群的范围为100–4,673 bp，平均为665 bp。具有大量读取的重叠群的功能注释主要与冷应答或光合作用有关。此外，鉴定了几种脂肪酸和TGA生物合成相关基因以及抗冻蛋白的推定同源物。进行了ArF0006与莱茵衣藻，金葡菌和拟南芥的比较，RT-PCR分析验证了生长温度对基因表达模式的影响。因此，ArF0006转录组分析提供了有关耐寒性的有用遗传信息，这些信息可能对生物工业应用有用。

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