...
首页> 外文期刊>Genetic Resources and Crop Evolution >Whole-genome analysis with SNPs from BOPA1 shows clearly defined groupings of Western Mediterranean, Ethiopian, and Fertile Crescent barleys
【24h】

Whole-genome analysis with SNPs from BOPA1 shows clearly defined groupings of Western Mediterranean, Ethiopian, and Fertile Crescent barleys

机译:使用BOPA1中的SNP进行全基因组分析显示了明确定义的西地中海,埃塞俄比亚和可育新月大麦的分组

获取原文
获取原文并翻译 | 示例
           

摘要

The discovery of Hordeum spontaneum C. Koch, a wild ancestor of cultivated barley, in Morocco in 1978 led to the proposal of a multicentric origin for this crop, as an alternative to the widely accepted theory of a single centre of domestication in the Fertile Crescent. Since this discovery, we have tested this hypothesis using the most advanced genetic techniques available at the time, from CM-proteins to RFLP and DNA-chloroplast markers. Nowadays, the availability of single nucleotide polymorphism (SNP) markers that are spread densely over the barley genome provides us with another powerful tool to give further support for the above. We have used 1,536 SNPs from the Barley Oligo Pool Assay 1 (BOPA1) of Illumina to characterize 107 wild and cultivated barley accessions from the Western Mediterranean, Fertile Crescent, Ethiopia, and Tibet. The results have confirmed that each location of the above-mentioned germplasm groups clusters separately. Analysis of molecular variance enabled us to focus on the chromosomal regions and loci that differentiated these groups of barley germplasm. Some of these regions contain vernalization and photoperiod response genes, some of the so-called domestication genes, as well as the most important quantitative trait locus for flowering time in the Mediterranean region. We have combined these results with other genetic evidence, and interpreted them in the framework of current theories on the onset of the Neolithic revolution in the Mediterranean region, to conclude that neither Ethiopia nor the Western Mediterranean can be ruled out as centres of barley domestication, together with the Fertile Crescent.
机译:1978年在摩洛哥发现了大麦的野生祖先大麦自发大麦C.科赫,因此提出了对该作物采用多中心起源的提议,作为广为接受的肥沃新月的单一驯化中心理论的替代方案。自从这一发现以来,我们已经使用当时可用的最先进的遗传技术(从CM蛋白到RFLP和DNA叶绿体标记物)验证了这一假设。如今,在大麦基因组上密集分布的单核苷酸多态性(SNP)标记的可用性为我们提供了另一个强大的工具,为上述工作提供了进一步的支持。我们已经使用了Illumina的大麦低聚池分析1(BOPA1)中的1,536个SNP,以表征来自西地中海,肥沃新月,埃塞俄比亚和西藏的107种野生和栽培大麦种质。结果证实上述种质组的每个位置分别成簇。分子差异分析使我们能够专注于区分这些大麦种质群体的染色体区域和基因座。这些区域中的一些包含春化和光周期反应基因,一些所谓的驯化基因,以及地中海地区开花时间最重要的数量性状基因座。我们将这些结果与其他遗传证据相结合,并在地中海地区新石器时代革命开始的当前理论框架下对其进行了解释,得出的结论是,不能排除埃塞俄比亚和西地中海是大麦驯化的中心,与肥沃的新月。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号