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首页> 外文期刊>Genetic Resources and Crop Evolution >Single-copy nuclear PolA1 gene sheds light on the origin of S genome with relationships to B and G genomes of polyploid wheat species
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Single-copy nuclear PolA1 gene sheds light on the origin of S genome with relationships to B and G genomes of polyploid wheat species

机译：单拷贝核PolA1基因揭示了S基因组的起源及其与多倍体小麦品种B和G基因组的关系

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摘要

PolA1, a single-copy nuclear gene encoding the largest subunit of RNA polymerase I, comprises highly polymorphic intron 19 and nucleotide tag (Ntag) sequences. We analyzed these sequences in 42 accessions, which differed in ploidy, of Triticum–Aegilops and Hordeum species. The lengths of the intron 19 sequences were ca. 110 bp long in Triticum–Aegilops species, except in four Sitopsis species, Ae. longissima, Ae. searsii, Ae. sharonensis, Ae. speltoides, which had introns similar in length to those of Hordeum species, i.e., ca. 240 bp long. Phylogenetic analyses of the Ntag sequences showed that the four Sitopsis and remaining Triticum–Aegilops species were classified into two discrete Hordeum and Triticum clades, respectively. The A and D genome-specific Ntag sequences of polyploid wheats were highly homologous with those of T. urartu and Ae. tauschii, respectively. In Ae. bicornis, another Sitopsis species, two accessions had the short intron 19 and Triticum–type Ntag sequence, which were highly homologous with those of the B genome in polyploid wheats, whereas one accession contained the long intron 19 and Hordeum–type Ntag sequences. In contrast, partial sequence analyses revealed that the three accessions of Ae. bicornis shared highly homology to single-copy DMC1 and EF-G genes. The discrepancy between these results indicates that the Sitopsis species were probably established by hybrid speciation including ancient introgressive hybridization between progenitors of Triticum–Aegilops and Hordeum. Although many researchers have proposed Ae. speltoides as a candidate for the B genome donor, our data suggest the existence of diploid B genome species in the past that were responsible for the origin of both polyploid wheats and Sitopsis species, including Ae. speltoides.
机译：PolA1是编码RNA聚合酶I最大亚基的单拷贝核基因，包含高度多态的内含子19和核苷酸标签（Ntag）序列。我们分析了42种小麦（小麦，黑麦和大麦）的倍性不同的这些序列。内含子19序列的长度为约2。 Triticum–Aegilops物种中110 bp长，除了4个Sitopsis物种Ae。 A. Longissima。西西里岛莎朗，Ae。 speltoides，其内含子的长度与大麦属（Sordeum）种的内含子相似。 240 bp长。对Ntag序列进行的系统进化分析表明，四种希托普氏菌和其余的小麦-盾木种类分别分为两个离散的大麦和小麦。多倍体小麦的A和D基因组特异性Ntag序列与T. urartu和Ae高度同源。陶西奇分别。在大江bicornis是另一个Sitopsis物种，两个登录号的内含子19和Triticum型Ntag短，与多倍体小麦B基因组的同源性很高，而一个登录号则包含长的内含子19和Hordeum型Ntag序列。相反，部分序列分析揭示了Ae的三个种。双角兽与单拷贝DMC1和EF-G基因具有高度同源性。这些结果之间的差异表明，Sitopsis物种可能是通过杂种形成的，包括古小麦-埃吉洛普斯和大麦的祖先之间的渐渗杂交。尽管许多研究人员提出了Ae。 speltoides作为B基因组供体的候选者，我们的数据表明过去存在二倍体B基因组物种，这是多倍体小麦和Sitopsis物种（包括Ae）起源的原因。 speltoides。

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