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首页> 外文期刊>Genetic Resources and Crop Evolution >Detection of genetic integrity of conserved maize (Zea mays L.) germplasm in genebanks using SNP markers
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Detection of genetic integrity of conserved maize (Zea mays L.) germplasm in genebanks using SNP markers

机译：使用SNP标记检测基因库中保守玉米（Zea mays L.）种质的遗传完整性

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摘要

Twenty maize landrace accessions regenerated and conserved in five maize genebanks were investigated for genetic integrity using 1,150 Single Nucleotide Polymorphisms (SNPs) and 235 SNP haplotypes. The genetic diversity of three accessions changed significantly in terms of the average number of alleles per locus. Ten out of twenty accessions had significantly different SNP allelic frequencies, either after regeneration or in the same accession held in different genebanks. The proportion of loci with significant changes in SNP allelic frequency was very low (37/1,150). Changes in the major allelic frequency (MAF) for the majority of SNP loci (60.2–75.2%) were less than 0.05. For SNP haplotypes, the genetic diversity of four accessions changed significantly in terms of average number of haplotype alleles and polymorphic information content (PIC) per locus. The proportion of SNP haplotype alleles lost in the later generations ranged between 0 and 22.6%, and at the same time 0–19.9% of the SNP haplotype alleles appeared in later generations, however, these were absent in the earlier generations. Dynamic changes in genetic integrity, in terms of presence and absence of genes (alleles), by both SNP and SNP haplotype analysis were detected during regeneration. A suboptimum number of ears harvested in one generation can be combined with those from another, repeated regeneration to capture the diversity of the previous generation. Use of molecular markers during regeneration of accessions can help in understanding the extent of genetic integrity of the maize accessions in ex situ genebanks and in recommending the best practice for maintaining the original genetic diversity of the genebank accessions.
机译：使用1,150个单核苷酸多态性（SNP）和235个SNP单倍型研究了五个玉米基因库中20个再生并保存的玉米地方品种的遗传完整性。就每个基因座的平均等位基因数目而言，三个种质的遗传多样性发生了显着变化。在再生后或保存在不同基因库中的同一登录物中，二十个登录物中有十个具有显着不同的SNP等位基因频率。 SNP等位基因频率发生显着变化的基因座比例非常低（37 / 1,150）。大多数SNP位点（60.2–75.2％）的主要等位基因频率（MAF）的变化小于0.05。对于单核苷酸多态性单倍型，四个种质的遗传多样性在单倍型等位基因的平均数目和每个基因座的多态信息含量（PIC）方面发生了显着变化。在后代中丢失的SNP单倍型等位基因的比例在0到22.6％之间，同时，在后代中出现了0–19.9％的SNP单倍型等位基因，但是在前几代中却不存在。在再生过程中，通过SNP和SNP单倍型分析检测到基因完整性的动态变化，无论是否存在基因（等位基因）。可以将一代中收获的次优穗数与另一次中重复收获的穗数相结合，以捕获上一代的多样性。在种质再生过程中使用分子标记可以帮助了解异地种质库中玉米种质的遗传完整性，并推荐最佳实践以维持种质库原种的遗传多样性。

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