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首页> 外文期刊>Genetic Resources and Crop Evolution >Inferring geographic origin of barley (Hordeum vulgare L. subsp. vulgare) accessions using molecular markers
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Inferring geographic origin of barley (Hordeum vulgare L. subsp. vulgare) accessions using molecular markers

机译：使用分子标记推断大麦（Hordeum vulgare L. subsp。vulgare）品种的地理起源

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The USDA Agricultural Research Service (ARS) National Small Grains Collection (NSGC) has 207 landrace barleys obtained from a nursery grown in the Ukraine in 1930 by N.I. Vavilov, many of which have multiple resistance (MR) to disease similar to accessions from Ethiopia. The original collection locations of the accessions from the Vavilov nursery are unknown. The purpose of this study was to determine, using molecular markers, if the MR accessions of unknown origin from the Vavilov nursery are genetically related to MR accessions from Ethiopia. The genetic relatedness among susceptible accessions of unknown origin and among a selection of landrace accessions from the NSGC barley core subset was also assessed. Simple sequence repeat (SSR) and Diversity Array Technology PL (DArT) marker data were used to generate similarity matrices with Dice’s similarity coefficients. Cluster analysis from these results showed that the unknown-origin MR accessions grouped with accessions from Ethiopia. Susceptible accessions of unknown origin were genetically similar to accessions from western Asia, especially near the Caucasus, and from both southern and northern Europe. Based on similarity of marker profiles, some accessions from the core collection are likely duplicates. Future work will seek to identify the probable origin of the remainder of the unknown origin accessions and to more thoroughly characterize the genetic diversity within NSGC barley core subset.
机译：美国农业部农业研究局（ARS）国家小谷物收藏（NSGC）拥有207个地方大麦，这些大麦是1913年由N.I从乌克兰的一个苗圃中获得的。瓦维洛夫（Vavilov），其中许多人对与埃塞俄比亚种质相似的疾病具有多重抵抗力（MR）。 Vavilov苗圃的种质原始收集地点未知。这项研究的目的是使用分子标记物来确定来自Vavilov苗圃的未知来源的MR病种是否与埃塞俄比亚的MR病种遗传相关。还评估了未知来源的易感种质之间以及来自NSGC大麦核心子集的一系列地方种质之间的遗传相关性。简单序列重复（SSR）和多样性阵列技术PL（DArT）标记数据用于生成具有Dice相似系数的相似矩阵。从这些结果进行的聚类分析表明，起源不明的MR病种与埃塞俄比亚的病种分组在一起。未知来源的易感种质在遗传上与西亚特别是高加索地区以及南欧和北欧的种质相似。基于标记图谱的相似性，来自核心馆藏的一些登录品可能是重复的。未来的工作将寻求确定未知来源种质的其余部分的可能来源，并更全面地表征NSGC大麦核心子集内的遗传多样性。

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