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首页> 外文期刊>Genetic Resources and Crop Evolution >Genetic diversity in Chinese sorghum landraces revealed by chloroplast simple sequence repeats
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Genetic diversity in Chinese sorghum landraces revealed by chloroplast simple sequence repeats

机译：叶绿体简单序列重复揭示中国高粱地方品种的遗传多样性

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A collection of sorghum, including more than 12,000 Chinese landraces, has been constructed and maintained in China. However, the genetic diversity of Chinese sorghum landraces has not been fully investigated, and the origin of Chinese sorghum is still in dispute. In this study, the complete chloroplast genome sequence of sorghum line Tx623B was searched for simple sequence repeats (SSRs). 31 SSR loci with at least 10 mononucleotide repeats or five dinucleotide repeats were identified, and primer pairs for 27 loci were designed. Chloroplast DNA variation in cultivated sorghum was investigated by using these primer pairs on 185 Chinese sorghum landraces and 70 cultivated sorghum accessions from other countries. Among the 27 loci, 14 were polymorphic. The number of alleles per polymorphic locus ranged from 2 to 5 with an average of 2.79. Allelic data at 14 polymorphic loci were combined to give 12 haplotypes. The average allelic diversity index across the 14 polymorphic loci and corresponding haplotype diversity were markedly lower for Chinese sorghum landraces than were those for accessions from other countries. However, Chinese sorghum landraces shared a predominant allele at each polymorphic locus and a predominant haplotype with foreign accessions. Our results indicate that Chinese landraces experienced a severe maternal bottleneck during the introduction process with a predominant haplotype being present in 171 of 185 accessions analyzed. Except for one rare exception, haplotypes found in Chinese landraces were either identical or closely related to those found in foreign accessions and could not be separated clearly from them by cluster analysis. Our results are consistent with the hypothesis of African origin of Chinese sorghum. Keywords Chloroplast - Genetic diversity - Kaoliang - Microsatellite - Origin - Sorghum bicolor
机译：在中国已经建造并维护了一批高粱，其中包括12,000多个中国地方品种。但是，中国高粱地方品种的遗传多样性尚未得到充分研究，中国高粱的起源仍存在争议。在这项研究中，搜索了高粱系Tx623B的完整叶绿体基因组序列，以寻找简单的序列重复序列（SSR）。鉴定出具有至少10个单核苷酸重复或5个二核苷酸重复的31个SSR基因座，并设计了27个基因座的引物对。利用这些引物对对185个中国高粱地方品种和70个其他国家的栽培高粱种质进行了研究，研究了这些栽培种高粱的叶绿体DNA变化。在27个基因座中，有14个是多态的。每个多态性基因座的等位基因数目范围为2至5，平均为2.79。将在14个多态位点的等位基因数据合并，得到12个单倍型。高粱地方品种的14个多态性位点的平均等位基因多样性指数和相应的单倍型多样性显着低于其他国家的种质。然而，中国高粱的地方品种在每个多态性位点共有一个主要的等位基因，并带有外来种质的一个主要单倍型。我们的结果表明，在引入的185个种质中，有171个种在引入过程中经历了严重的母本瓶颈。除了一个罕见的例外，在中国地方品种中发现的单倍型与在国外种质中发现的单倍型相同或紧密相关，并且不能通过聚类分析将其与它们分开。我们的结果与非洲起源于中国高粱的假说相符。关键词叶绿体-遗传多样性-高粱-微卫星-起源-高粱

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