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首页> 外文期刊>Genetic Resources and Crop Evolution >Genic SSRs for European and North American hop (Humulus lupulus L.)
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Genic SSRs for European and North American hop (Humulus lupulus L.)

机译：欧洲和北美啤酒花（Humulus lupulus L.）的基因SSR

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摘要

Eight genic SSR loci were evaluated for genetic diversity assessment and genotype identification in Humulus lupulus L. from Europe and North America. Genetic diversity, as measured by three diversity indices, was significantly lower in European cultivars than in North American wild accessions. Neighbor Joining cluster analysis separated the hop genotypes into European and North American groups. These eight SSRs were useful in uniquely identifying each accession with the exception of two sets of European landraces and a pair of Japanese cultivars, ‘Shinshuwase’ and ‘Kirin II’. An accession from Manitoba grouped with the European (EU) cluster reflecting the group’s genetic similarity to older Manitoba germplasm used to develop ‘Brewer's Gold’ and the gene pool arising from this cultivar. Cultivars grouped closely with one of their immediate parents. ‘Perle’ grouped with its parent ‘Northern Brewer and ‘Willamette’ grouped with its parent ‘Fuggle H’. Wild American accessions were divided into two subgroups: a North Central group containing mostly H. lupulus var. lupuloides and a Southwestern group containing H. lupulus var. neomexicanus accessions. These eight SSRs will be valuable for genotype identification in European and wild American germplasm and may potentially prove useful for marker-assisted selection in hop. PCR products from four previously reported primer pairs that amplify the same intronic SSR regions as do the genic SSRs in this study were compared in eight common cultivars. Different primer pairs generated robust markers at the chs2 and chi loci. However, only the HLC-004B and HLC-006 primer pairs amplified successfully at the chs3 and chs4 loci.
机译：对来自欧洲和北美的Hum草中的八个基因SSR基因座进行了遗传多样性评估和基因型鉴定。用三个多样性指数衡量，欧洲品种的遗传多样性显着低于北美野生种。邻居加入聚类分析将啤酒花基因型分为欧洲和北美组。这八种SSR对唯一鉴定每个种质很有用，除了两套欧洲地方品种和一对日本品种“ Shinshuwase”和“ Kirin II”外。曼尼托巴省的一个与欧洲（EU）聚簇在一起的种质反映了该组织与用于开发“酿酒师的黄金”的曼尼托巴省较旧种质的遗传相似性，以及该品种产生的基因库。品种与他们的直系亲属紧密合在一起。 “珀尔”与父级“北方啤酒”分组，而“维拉米特”与父级“ Fuggle H”分组。美国野生种被分为两个亚群：北中部群，主要含有H. lupulus var。七倍体和西南组含有H. lupulus var。新墨西哥犬种。这八个SSR对于欧洲和美洲野生种质的基因型鉴定将是有价值的，并且可能被证明对啤酒花的标记辅助选择有用。在八个普通品种中，对四个先前报道的引物对的PCR产物进行了比较，这些引物对扩增了与本研究中的基因SSR相同的内含子SSR区域。不同的引物对在chs2和chi位点产生了强大的标记。但是，只有HLC-004B和HLC-006引物对在chs3和chs4位点成功扩增。

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