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Phylogenetic relationships within Citrus and its related genera as inferred from AFLP markers

机译:从AFLP标记推断的柑橘及其相关属的系统发生关系

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摘要

We investigated the phylogenetic relationships among Citrus and its relatives, including 29 genotypes belonging to Citrus, Poncirus, Fortunella, Microcitrus, Eremocitrus, Atalantia and Severinia using AFLP analysis. Cluster analysis was conducted using neighbour joining (NJ) method. The results demonstrated that Poncirus, Microcitrus and Eremocitrus are distant from Citrus. A strong affiliation exists between C. halimii and Fortunella and the results did not support C. halimii as the fourth basic species. Further evidence was provided that P. polyandra should not be the hybrid between trifoliate orange and Citrus genotypes and it deserves species status. Neither the separation of subgenus Papeda and Citrus nor the separation of subgenus Archicitrus and Metacitrus were clearly resolved in the dendrogram. C. ichangesis is a distinct species very different from other Citrus genotypes, and the results showed it is improper to classify it into subgenus Metacitrus. C. reticulata, C. maxima and C. medica were separated into three distinct clusters. Therefore if only cultivated Citrus species are considered, the three basic species concept is acceptable. However, it is not applicable for whole genus Citrus.
机译:我们使用AFLP分析法研究了柑橘及其亲属之间的系统发育关系,包括29种基因型,分别属于柑橘,poncirus,Fortunella,Microcitrus,Eremocitrus,Atalantia和Severinia。使用邻居加入(NJ)方法进行聚类分析。结果表明,柑桔,微柑桔和Er柑离柑橘较远。哈里木梭菌和福门氏菌之间存在很强的隶属关系,其结果不支持哈里木梭菌作为第四基本物种。进一步的证据表明,P。polyandra不应该是三叶形橙和柑橘基因型之间的杂种,它应该具有物种地位。树状图没有清楚地分辨出Papeda和柑橘属的分离以及Archicitrus和Metacitrus亚属的分离。 C. ichangesis是一个与其他柑橘基因型非常不同的独特物种,结果表明将其分类为Metacitrus亚属是不适当的。网状隐孢子虫,最大隐孢子虫和药用隐孢子虫被分为三个不同的簇。因此,如果仅考虑栽培柑橘品种,则可以接受三个基本物种概念。但是,它不适用于整个柑橘属。

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