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首页> 外文期刊>Genetic Resources and Crop Evolution >Gene expression variation in grapevine species Vitis vinifera L. and Vitis aestivalis Michx.
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Gene expression variation in grapevine species Vitis vinifera L. and Vitis aestivalis Michx.

机译：葡萄树葡萄和葡萄树Michx的基因表达差异。

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摘要

Comparative microarray (Vitis GeneChip) analysis in young leaves revealed considerable variation in gene expression between Vitis vinifera L. and Vitis aestivalis Michx. Approximately 12% of the genes were differentially expressed in the two grapevine species (P < 0.001). Over 200 probe sets were identified which consistently detected transcripts in one grapevine species, but not in the other. We were unable to identify any broad functional category in which transcript abundance was overall different in any one species. Of the genes expressed only in V. aestivalis leaves, we identified a class IV chitinase which was previously shown by others to have a flower- and fruit-specific expression in V. vinifera. Among the transcripts which were differentially expressed (P < 0.001) in both species, we identified genes encoding key enzymes in flavonoid, monolignol, and proanthocyanindin biosynthesis. Statistical exploration of the data suggested that sequence divergence between the predominantly V. vinifera-derived GeneChip probes and the V. aestivalis cRNA did not confound the hybridization data and that the reliability of the microarray results was similar in the two grapevine species.
机译：幼叶中的比较微阵列（Vitis GeneChip）分析表明，Vitis vinifera L.和Vitis aestivalis Michx之间的基因表达存在相当大的差异。两种葡萄中大约有12％的基因差异表达（P <0.001）。鉴定出200多个探针组，它们在一个葡萄树种中始终检测到转录本，而在其他葡萄树种中未检测到。我们无法确定在任何一个物种中转录本丰度总体上都不同的任何广泛的功能类别。在仅在V. aestivalis叶子中表达的基因中，我们鉴定了IV类几丁质酶，该酶先前已被其他人证明在V. vinifera中具有花和果特异性表达。在两个物种中差异表达的转录本中（P <0.001），我们鉴定了在类黄酮，单木酚和原花青素生物合成中编码关键酶的基因。对数据的统计探索表明，主要来自酿酒葡萄的GeneChip探针和酿酒酵母cRNA之间的序列差异并未混淆杂交数据，并且在两个葡萄品种中，微​​阵列结果的可靠性相似。

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