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首页> 外文期刊>Genetics >Components of the Spindle Assembly Checkpoint Regulate the Anaphase-Promoting Complex During Meiosis in Caenorhabditis elegans
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Components of the Spindle Assembly Checkpoint Regulate the Anaphase-Promoting Complex During Meiosis in Caenorhabditis elegans

机译：秀丽隐杆线虫减数分裂期间主轴装配检查点的成分调节后期促进复合物。

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Temperature-sensitive mutations in subunits of the Caenorhabditis elegans anaphase-promoting complexn(APC) arrest at metaphase of meiosis I at the restrictive temperature. Embryos depleted of the APC coactivatornFZY-1 by RNAi also arrest at this stage. To identify regulators and potential substrates of the APC, wenperformed a genetic suppressor screen with a weak allele of the APC subunit MAT-3/CDC23/APC8, whosendefects are specific to meiosis. Twenty-seven suppressors that resulted in embryonic viability and larvalndevelopment at the restrictive temperature were isolated. We have identified the molecular lesions in 18 ofnthese suppressors, which correspond to five genes. In addition to a single intragenic suppressor, we foundnmutations in the APC co-activator fzy-1 and in three spindle assembly checkpoint genes, mdf-1, mdf-2, and mdf-n3/san-1, orthologs of Mad1, Mad2, and Mad3, respectively. Reduction-of-function alleles of mdf-2 and mdf-3nsuppress APC mutants and exhibit pleiotropic phenotypes in an otherwise wild-type background. Analysis ofna single separation-of-function allele of mdf-1 suggests that MDF-1 has a dual role during development. Thesenstudies provide evidence that components of the spindle assembly checkpoint may regulate the metaphaseto-nanaphase transition in the absence of spindle damage during C. elegans meiosis.
机译：秀丽隐杆线虫后期促进复合物（APC）亚基的温度敏感突变在限制性温度下在减数分裂I的中期停滞。 RNAi耗尽了APC共激活因子nFZY-1的胚胎也在这一阶段停滞。为了鉴定APC的调控因子和潜在底物，对APC亚基MAT-3 / CDC23 / APC8等位基因的弱等位基因进行了基因抑制，并对其进行了鉴定。分离了27种在限制性温度下可导致胚胎存活和幼虫发育的抑制剂。我们已经鉴定出18种抑制因子的分子损伤，它们与5个基因相对应。除了单个基因内抑制子外，我们还发现了APC共激活因子fzy-1和三个纺锤体装配检查点基因mdf-1，mdf-2和mdf-n3 / san-1，Mad1，Mad2直系同源基因，和Mad3分别。 mdf-2和mdf-3ns的功能减少等位基因抑制APC突变体，并在其他野生型背景下表现出多效性表型。对mdf-1的单个功能分离等位基因进行分析表明，MDF-1在发育过程中具有双重作用。这些研究提供了证据表明，在秀丽隐杆线虫减数分裂过程中，在没有纺锤体损害的情况下，纺锤体装配检查点的组件可能调节了从中期到后期的转变。

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