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首页> 外文期刊>Harmful Algae >Membrane-like protein involved in phage adsorption associated with phage-sensitivity in the bloom-forming cyanobacterium Microcystis aeruginosa
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Membrane-like protein involved in phage adsorption associated with phage-sensitivity in the bloom-forming cyanobacterium Microcystis aeruginosa

机译：膜样蛋白参与噬菌体的吸附，与形成水华的蓝藻微囊藻中的噬菌体敏感性有关

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摘要

The cyanobacterium, Microcystis aeruginosa, contains a large number of defense genes (Makarova et al., 2011); thus, it is a good model to study the co-evolution of phage and bacteria. Here, we isolated and characterized two phage-resistant M. aeruginosa mutants that came from a phage intermediate-sensitive culture. To determine the mutation conferring resistance, a protein expression pattern analysis was performed comparing phage-sensitive and -resistant sub-strains using SDS-PAGE. There were no apparent differences in expression patterns in the soluble fraction; however, a ～90 kDa protein in the hydrophobic fraction from the phage-sensitive sub-strain was observed. Using a successive thermal asymmetric interlaced-PCR, the entire sequence encoding the protein, assigned ISP90, as well as its neighboring regions (ca. 7.8 kb) was determined. ISP90 contained no conserved domains and was predicted to be a membrane-associated protein. No mutations were detected in the nucleotide sequences coding ISP90 and diversification of ISP90 regions within this species were observed. Diversification of ISP90 regions within this species suggests a possible genomic island that may be subjected to selective pressures from phages. The ISP90 sequence involving phage resistance/sensitivity contributes to the understanding of co-evolution between M. aeruginosa and phages.
机译：蓝细菌铜绿微囊藻含有大量防御基因（Makarova等，2011）；因此，它是研究噬菌体与细菌共同进化的良好模型。在这里，我们分离并鉴定了来自噬菌体中间敏感培养物的两个噬菌体抗性铜绿假单胞菌突变体。为了确定赋予抗性的突变，使用SDS-PAGE比较了噬菌体敏感和抗性亚菌株，进行了蛋白质表达模式分析。可溶级分的表达方式没有明显差异。然而，在噬菌体敏感亚菌株的疏水部分中观察到了约90 kDa的蛋白质。使用连续的热不对称交错式PCR，确定了编码蛋白质的整个序列（分配为ISP90）及其邻近区域（约7.8 kb）。 ISP90不包含保守结构域，并且被认为是一种膜相关蛋白。在编码ISP90的核苷酸序列中未检测到突变，并且在该物种内未观察到ISP90区域的多样化。该物种内ISP90区域的多样化表明可能存在一个基因组岛，可能受到噬菌体的选择性压力。涉及噬菌体抗性/敏感性的ISP90序列有助于理解铜绿假单胞菌和噬菌体之间的共同进化。

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  • 来源
    《Harmful Algae》 |2014年第4期|69-75|共7页
  • 作者

    Takashi Yoshida; Rikae Kamiji; Ginji Nakamura; Takakazu Kaneko; Yoshihiko Sako;

  • 作者单位

    Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyoku, Kyoto 606-8502, Japan;

    Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyoku, Kyoto 606-8502, Japan;

    Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyoku, Kyoto 606-8502, Japan;

    Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-Ku, Kyoto 603-8555, Japan;

    Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyoku, Kyoto 606-8502, Japan;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Cyanobacteria; Microcystis aeruginosa; Cyanophage; Phage resistance;

    机译：蓝细菌;铜绿微囊藻;噬菌体;噬菌体抗性;

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