High throughput sequencing of 18S rRNA and its gene to characterize a Prorocentrum shikokuense (Dinophyceae) bloom

Zhang Yaqun; Lin Xin; Li Tangcheng; Li Hengde; Lin Lingxiao; Luo Hao; Li Ling; Ji Nanjing; Lin Senjie

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High throughput sequencing of 18S rRNA and its gene to characterize a Prorocentrum shikokuense (Dinophyceae) bloom

机译：高通量测序18s rRNA及其基因表征序列（Dinophyceae）绽放

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Comparing phytoplankton non-bloom and bloom communities using rRNA and its coding gene can help understand the shift of dominant species and its driving processes (e.g., intrinsic growth or grazing). Here we conducted high-throughput sequencing of 18S rRNA and its coding gene for studying non-bloom and bloom plankton communities in East China Sea. The non-bloom community was dominated by diatoms whereas during the bloom it was dominated by the dinoflagellate Prorocentrum shikokuense (formerly P. donghaiense). P. shikokuense rRNA abundance and rRNA:rRNA gene ratio both increased markedly in the bloom, indicating that the bloom arose from active growth. In contrast, some non-bloom species showed low DNA abundances during the bloom albeit high rRNA:rRNA gene ratios, suggesting that cell loss processes such as grazing might have prevented these species from blooming or that these species might be at an early stage of bloom development. Furthermore, Pearson's correlation analysis showed that dinoflagellate abundance was positively correlated with temperature and negatively related to dissolved inorganic phosphate (DIP) concentrations, suggesting warm and DIP-poor environment as a niche space for P. shikokuense. Our results demonstrate the usefulness of combined analysis of rRNA and its gene in characterizing phytoplankton bloom development to shed light on the complex phytoplankton dynamics and regulating mechanisms in the course of bloom development.

机译：使用RRNA的Phytoplankton非绽放和盛开社区进行比较，其编码基因可以帮助了解优势物种的转变及其驱动过程（例如，内在生长或放牧）。在这里，我们进行了高通量测序18S rRNA及其编码基因，用于研究东海的非盛开和盛开浮游生物社区。非绽放社区由硅藻占主导，而在盛开期间，它是由Dinoflagellate Prorocentrum Shikokuense（以前P. Dongaiense）的占主导地位。 P. shikokuense rRNA丰度和rRNA：勃起的rRNA基因比例在盛开中显着增加，表明盛开从积极的增长中产生。相比之下，一些非绽放物种在盛开期间显示出低DNA丰度，虽然高rRNA：rRNA基因比率，表明诸如放牧的细胞损失过程可能会阻止这些物种盛开，或者这些物种可能是盛开的早期阶段发展。此外，Pearson的相关性分析表明，Dinoflagelate丰度与溶解无机磷酸盐（DIP）浓度的温度呈正相关，暗示温暖和潜入的环境作为P.Shikokuense的利基空间。我们的结果表明，rRNA及其基因在特征浮游植物的植物发育中的综合分析和其基因的有用性在盛开的发展过程中对复杂的浮游植物动力学和调节机制进行了揭示。

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来源
《Harmful Algae》 |2020年第4期|101809.1-101809.11|共11页
作者
Zhang Yaqun; Lin Xin; Li Tangcheng; Li Hengde; Lin Lingxiao; Luo Hao; Li Ling; Ji Nanjing; Lin Senjie;
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Xiamen Univ Coll Ocean & Earth Sci State Key Lab Marine Environm Sci Xiamen 361102 Peoples R China|Chinese Acad Fishery Sci Key Lab Aquat Genom Beijing Key Lab Fishery Biotechnol Minist Agr & Rural Affairs Beijing 100141 Peoples R China;

Xiamen Univ Coll Ocean & Earth Sci State Key Lab Marine Environm Sci Xiamen 361102 Peoples R China;

Xiamen Univ Coll Ocean & Earth Sci State Key Lab Marine Environm Sci Xiamen 361102 Peoples R China;

Chinese Acad Fishery Sci Key Lab Aquat Genom Beijing Key Lab Fishery Biotechnol Minist Agr & Rural Affairs Beijing 100141 Peoples R China;

Xiamen Univ Coll Ocean & Earth Sci State Key Lab Marine Environm Sci Xiamen 361102 Peoples R China;

Xiamen Univ Coll Ocean & Earth Sci State Key Lab Marine Environm Sci Xiamen 361102 Peoples R China;

Xiamen Univ Coll Ocean & Earth Sci State Key Lab Marine Environm Sci Xiamen 361102 Peoples R China;

Xiamen Univ Coll Ocean & Earth Sci State Key Lab Marine Environm Sci Xiamen 361102 Peoples R China;

Xiamen Univ Coll Ocean & Earth Sci State Key Lab Marine Environm Sci Xiamen 361102 Peoples R China|Xiamen Univ Xiamen Key Lab Urban Sea Ecol Conservat & Restora Xiamen 361102 Peoples R China|Univ Connecticut Dept Marine Sci Groton CT 06405 USA;

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正文语种 eng
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关键词
rRNA gene; rRNA:rRNA gene ratio; Dinoflagellate bloom; Community diversity; East China Sea, Prorocentrum shikokuense;

机译：rRNA基因;rRNA：rRNA基因比例;Dinoflagellate Bloom;社区多样性;东海;普罗伦敦省夏科邦;

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High throughput sequencing of 18S rRNA and its gene to characterize a Prorocentrum shikokuense (Dinophyceae) bloom

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