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首页> 外文期刊>ICES Journal of Marine Science >Bacteria in the gut of juvenile cod Gadus morhua fed live feed enriched with four different commercial diets
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Bacteria in the gut of juvenile cod Gadus morhua fed live feed enriched with four different commercial diets

机译：幼鳕鱼肠道中的细菌饲喂了富含四种不同商业饮食的活饲料

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摘要

Atlantic cod, Gadus morhua L., larvae were fed rotifers, Brachionus plicatilis and Artemia franciscana enriched on four different commercial media, using the manufacturers' protocols. Pooled samples of 20 cod larvae were homogenized, diluted, and plated out on Petri dishes. The number of colony-forming units per larva was estimated, and the dominant strains subsequently sampled for sequencing of 16S rDNA. Bacteria showing high sequence similarity to a pathogen characteristic of cod and other fish species, Listonella anguillarum, were present in all four groups. Other taxa present among the dominating bacterial colonies were Pseudoalteromonas sp., and Vibrio sp. However, these bacteria could be assigned to genera only. The different enrichments probably affected the number of colony-forming bacteria per millilitre in the enrichment cultures as well as in the larval gastrointestinal (GI) tract. Also, the composition of the microbiota associated with the larval GI tract was probably affected by the enrichment media.
机译：按照制造商的规定，在四种不同的商业培养基上给大西洋鳕，Gadus morhua L.，幼虫饲喂富含四种不同商业培养基的轮虫，褶皱小臂and和Art蒿。将20只鳕鱼幼虫的合并样品匀浆，稀释并铺在培养皿上。估计每个幼虫的菌落形成单位数，随后取样优势菌株以进行16S rDNA测序。在所有四个组中均存在与鳕鱼和其他鱼类鳗鱼李斯特氏菌的病原体具有高度序列相似性的细菌。存在于主要细菌菌落中的其他分类群是假单胞菌属种和弧菌属种。但是，这些细菌只能分配给属。不同的富集可能会影响富集培养物中以及幼虫胃肠道中每毫升菌落形成细菌的数量。同样，与幼虫胃肠道有关的微生物群的组成可能受到富集培养基的影响。

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