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Extraction of Blebs in Human Embryonic Stem Cell Videos

机译:人类胚胎干细胞视频中小疱的提取

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摘要

Blebbing is an important biological indicator in determining the health of human embryonic stem cells (hESC). Especially, areas of a bleb sequence in a video are often used to distinguish two cell blebbing behaviors in hESC: dynamic and apoptotic blebbings. This paper analyzes various segmentation methods for bleb extraction in hESC videos and introduces a bio-inspired score function to improve the performance in bleb extraction. Full bleb formation consists of bleb expansion and retraction. Blebs change their size and image properties dynamically in both processes and between frames. Therefore, adaptive parameters are needed for each segmentation method. A score function derived from the change of bleb area and orientation between consecutive frames is proposed which provides adaptive parameters for bleb extraction in videos. In comparison to manual analysis, the proposed method provides an automated fast and accurate approach for bleb sequence extraction.
机译:抽血是确定人类胚胎干细胞(hESC)健康的重要生物学指标。特别是,视频中气泡序列的区域通常用于区分hESC中的两种细胞气泡行为:动态和细胞凋亡。本文分析了hESC视频中气泡提取的各种分割方法,并介绍了一种具有生物启发性的评分功能,以提高气泡提取的性能。完整的气泡形成包括气泡扩展和收缩。小球在两个过程中以及在帧之间都动态更改其大小和图像属性。因此,每种分割方法都需要自适应参数。提出了从连续区域之间的气泡面积和方向变化得出的得分函数,该函数为视频中的气泡提取提供了自适应参数。与手动分析相比,该方法为气泡序列提取提供了一种自动,快速,准确的方法。

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