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首页> 外文期刊>Indian Journal of Microbiology >Molecular Diversity and Mating Type Distribution of the Rice Blast Pathogen Magnaporthe oryzae in North-East and Eastern India
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Molecular Diversity and Mating Type Distribution of the Rice Blast Pathogen Magnaporthe oryzae in North-East and Eastern India

机译：印度东北部和东部印度稻瘟病菌稻瘟病菌的分子多样性和交配类型分布

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Rice blast, caused by Magnaporthe oryzae, is the most devastating disease of rice and severely affects crop stability and sustainability worldwide. In this study, a total of 63 single spore isolates, collected during 2010–2013 from different cultivars in the different rice growing regions of North-East and Eastern India were used for molecular diversity and mating type analysis. DNA fingerprinting was used to study the diversity among the collections of 63 isolates by using POT2-TIR rep-PCR and MGR586-TIR. Different lineages were detected for 63 M. oryzae isolates by Pot2-TIR and eight for MGR586-TIR fingerprints at 75 % similarity. Among the lineages detected by Pot2-TIR, lineage A and I represented the maximum number of isolates whereas other lineages represent fewer numbers of isolates. Generally all the lineages contained isolates of mixed geographical origin. Isolates from Jharkhand were distributed in all the seven lineages. The MGR586-TIR DNA fingerprinting detected eight lineages, out of which three (Lineages F, G, H) were site specific but were represented only by single isolate. Lineage C contained isolates of Jharkhand only. The lineage A was the largest represented 46 isolates from all the states except Madhya Pradesh. Optimization of the sampling may result in considerable improvement in the results as clustering of isolates from Jharkhand in a few lineages and detection of different lineages with limited isolates from other states could be ascribed to improper sampling. MGR586-TIR fingerprints appeared to differentiate the isolates more strongly compared to POT2-TIR as is obvious from the distance among isolates of the same lineage (Lineage A) arbitrarily grouped together at 75 % similarity. All the 63 isolates were also investigated for MAT1-1 and MAT1-2 mating-type distribution by PCR based molecular markers. Of the 63 M. oryzae isolates collected, 16 (25 %) of the isolates were the mating type MAT1-1 while 35 (56 %) were mating type MAT1-2. The MAT1-2 isolates predominated in Jharkhand and Assam while MAT1-1 is more predominant in the isolates of Odisha. Both MAT1-1 and MAT1-2 were equally distributed in the isolates of Meghalaya and Tripura. Only single isolate from Jharkhand was positive for both the mating type. The results indicated that sexual recombination might be the one reason for lineage diversity in M. oryzae in fields of large rice-growing regions in North-East and Eastern states of India.
机译：由稻瘟病菌（Magnaporthe oryzae）引起的稻瘟病是最毁灭性的稻瘟病，严重影响着全球的作物稳定性和可持续性。在这项研究中，从2010年至2013年间从印度东北和印度东部不同水稻种植地区的不同品种中收集的63株单孢子分离株用于分子多样性和交配类型分析。通过POT2-TIR rep-PCR和MGR586-TIR，DNA指纹图谱用于研究63个分离株的多样性。 Pot2-TIR检测到63种米曲霉分离株的不同谱系，MGR586-TIR指纹图谱检测出8种，相似度为75％。在Pot2-TIR检测到的谱系中，谱系A和I代表了最大数量的分离株，而其他谱系则代表了较少的分离株。通常，所有谱系都包含混合地理来源的分离株。来自贾坎德邦的分离株分布在所有七个谱系中。 MGR586-TIR DNA指纹检测到八个谱系，其中三个（谱系F，G，H）是位点特异性的，但仅由单个分离株代表。谱系C仅包含贾坎德邦的分离株。 A谱系是最大的，代表了除中央邦以外所有州的46个分离株。采样的优化可能会导致结果的显着改善，因为来自贾坎德邦（Jharkhand）的分离株在几个谱系中聚集，并且检测到来自其他州的有限分离株的不同谱系可能归因于不正确的采样。与POT2-TIR相比，MGR586-TIR指纹图谱似乎更能区分分离株，这是从相同谱系（谱系A）的任意分离株之间以75％相似度任意分组的距离显而易见的。还通过基于PCR的分子标记对63个分离株的MAT1-1和MAT1-2交配型分布进行了研究。在收集的63种米曲霉菌株中，有16种（25％）为MAT1-1交配型，而35种（56％）为MAT1-2型交配。 MAT1-2分离株在贾坎德邦和阿萨姆邦占主导地位，而MAT1-1在奥里萨邦的分离株中占主导地位。 MAT1-1和MAT1-2在梅加拉亚邦和Tripura的分离物中均等分布。对于两种交配类型，仅来自贾坎德邦的分离株均为阳性。结果表明，有性重组可能是印度东北和东部大米种植区稻米中米曲霉谱系多样性的原因之一。

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