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首页> 外文期刊>International journal of food science & technology >Pilot-scale Protamex™-catalysed production of round scad protein hydrolysates: effects of agitation alone and combined with aeration
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Pilot-scale Protamex™-catalysed production of round scad protein hydrolysates: effects of agitation alone and combined with aeration

机译:Protamex™催化规模的圆形Scad蛋白水解产物的生产:单独搅拌和通气的效果

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摘要

A pilot-scale production of round scad protein hydrolysates via Protamex™-catalysed proteolysis was firstly established. Process optimisation then focused on the use of mechanical agitation (MA) alone or combined with aeration (MAA). Comparative examinations revealed that proteolysis involving either MA or MAA was feasible with a hydrolysis degree up to 55%, and individual and total biogenic amine (BA) contents much lower than the commonly recognised limits (including the dominant BA, cadaverine). MAA is more advantageous in suppressing bacterial counts and activities associated with protein decomposition, enabling reduced volatile basic nitrogen and BAs. Thus, additional aeration to mechanical agitation is recommended for industrial proteolysis of raw materials unavoidably containing micro-organisms (especially anaerobic bacteria) in common handling practice.
机译:首先建立了通过Protamex™催化的蛋白水解的圆形Scad蛋白质水解产物的中试规模。然后,工艺优化的重点是单独使用机械搅拌(MA)或与曝气(MAA)结合使用。对比检查表明,涉及MA或MAA的蛋白水解是可行的,水解度最高可达55%,并且单个和全部生物胺含量(BA)远低于公认的限度(包括显性BA,尸胺)。 MAA在抑制细菌数量和与蛋白质分解相关的活性方面更具优势,可以减少挥发性碱性氮和BA。因此,在通常的处理实践中,建议对机械搅拌附加通气,以对不可避免地包含微生物(尤其是厌氧细菌)的原料进行工业蛋白水解。

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