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首页> 外文期刊>International Journal of Heat and Mass Transfer >Study on Leidenfrost effect of cryoprotectant droplets on liquid nitrogen with IR imaging technology and non-isothermal crystallization kinetics model
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Study on Leidenfrost effect of cryoprotectant droplets on liquid nitrogen with IR imaging technology and non-isothermal crystallization kinetics model

机译：红外成像技术和非等温结晶动力学模型研究防冻剂液滴对液氮的莱顿弗罗斯特效应

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摘要

Droplet vitrification is one of the most attractive tools for cells cryopreservation due to its ultra-rapid cooling rate. However, this method has its own limitation because of the Leidenfrost phenomenon. In this work, the infrared imaging technology was utilized to investigate the effects of cryoprotective agents (CPA) concentrations and volumes on levitating velocities and durations of CPA droplets on liquid nitrogen (LN2), and the model of non-isothermal crystallization kinetics was also adopted here for theoretical analysis of the temperature distributions and degree of crystallization inside the droplets. The experimental results showed that the movements of droplets on LN2 became slower when increasing the CPA concentrations, but they were independent on the volumes except for higher CPA concentration cases (i.e., 50% glycerol and VS55). And the Leidenfrost effect time (i.e. the levitation time on the liquid nitrogen) became longer as increasing both the CPA concentrations and volumes. The theoretical results indicated that the final crystallinity inside droplets reached 1 for both water and 20% glycerol cases, a magnitude of 1 x 10(-6) for 50% glycerol and 1 x 10(-)(13) for VS55, respectively. Furthermore, the motion of droplets was strongly related to the crystallinity rates and cooling rates inside the droplets. (C) 2018 Elsevier Ltd. All rights reserved.
机译：由于其超快的冷却速度，液滴玻璃化是细胞冷冻保存最吸引人的工具之一。但是，由于莱顿弗罗斯特现象，该方法有其自身的局限性。在这项工作中，利用红外成像技术研究了冷冻保护剂（CPA）的浓度和体积对液氮（LN2）上悬浮液滴的悬浮速度和持续时间的影响，并且还采用了非等温结晶动力学模型在此对液滴内部的温度分布和结晶度进行理论分析。实验结果表明，当CPA浓度增加时，液滴在LN2上的运动变慢，但除了CPA浓度较高的情况（即50％甘油和VS55）外，它们与体积无关。随着CPA浓度和体积的增加，莱顿弗罗斯特效应时间（即液氮上的悬浮时间）变得更长。理论结果表明，对于水和20％的甘油，液滴内部的最终结晶度均达到1；对于50％的甘油，液滴的最终结晶度分别为1 x 10（-6）；对于VS55，其最终结晶度分别为1 x 10（-）（13）。此外，液滴的运动与液滴内部的结晶速率和冷却速率密切相关。 （C）2018 Elsevier Ltd.保留所有权利。

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