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首页> 外文期刊>journal of biomedical optics >Fiber-based combined optical coherence and multiphoton endomicroscopy
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Fiber-based combined optical coherence and multiphoton endomicroscopy

机译：基于光纤的光学相干和多光子内窥镜

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摘要

A fiber-based multimodal imaging system that combines multiphoton microscopy (MPM) with Fourierndomain optical coherence microscopy (OCM) is reported. The system uses a fiber-based femtosecond laser, a fiberncoupler, and a double-clad fiber (DCF) device. The fiber laser has a central wavelength of 1.04 μm and bandwidthnof 29 nm. Longer excitation wavelength is used to increase penetration depth and increase the excitation efficiencynfor dyes, such as red fluorescent dyes. A single mode fiber coupler is used to replace the free-space beam splitternand one arm of the coupler is fused with a double-clad fiber device. TheMPM and OCM share the same excitationnlight path in the core of a double-clad fiber, while the OCM and MPM signals were collected by the core andnclad of the double-clad fiber, respectively. The performance of the introduced double-clad device is analyzed.nThe device can confine all the excitation light in the core and has a collection efficiency of 20% for the MPMnsignal. The efficiency can be further increased by fusing more multimode fibers with the DCF. Simultaneousnoptical coherence microscopic imaging, second harmonic generation imaging, and two-photon excitation flu-norescence imaging are demonstrated in biological samples.
机译：报道了一种基于纤维的多峰成像系统，该系统将多光子显微镜（MPM）与傅里叶域光学相干显微镜（OCM）相结合。该系统使用基于光纤的飞秒激光器，光纤耦合器和双包层光纤（DCF）设备。光纤激光器的中心波长为1.04μm，带宽n为29 nm。较长的激发波长用于增加染料的渗透深度并提高激发效率，例如红色荧光染料。使用单模光纤耦合器来代替自由空间光束分离器，并且耦合器的一个臂与双包层光纤设备融合在一起。 MPM和OCM在双包层光纤的核心中共享相同的激发光路径，而OCM和MPM信号分别由双包层光纤的核心和边缘收集。分析了引入的双包层器件的性能。n该器件可以限制所有激发光在芯中，并且对MPMnsignal具有20％的收集效率。通过将更多的多模光纤与DCF融合，可以进一步提高效率。在生物样品中同时进行了相干相干显微成像，二次谐波生成成像和双光子激发荧光成像。

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