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Comparison of kjeldahl and dumas methods for determining protein contents of soybean products

机译：凯氏定氮法与杜马方法测定大豆制品蛋白质含量的比较

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摘要

The Kjeldahl and Dumas methods for quantifying nitrogen content were compared using nine soybean products having protein contents ranging from 0.5 to 90%. In addition to comparing day-to-day variability of the Dumas method, differences between and variabilities of two Kjeldahl systems and Kjeldahl operators were also evaluated. The Kjeldahl method gave slightly, but significantly, lower values than did the Dumas method. Both the Kjeldahl and Dumas methods had equivalent variabilities (same SD about the means). The ratios between the means for the Kjeldahl and Dumas (K/D) protein values ranged from 0.66 to 1.03. The conversion equation y=−0.00536+0.97188x (R 2=0.9997) was developed and validated to convert from Dumas to kjeldahl protein concentrations.
机译：使用九种大豆蛋白含量为0.5％至90％的大豆产品，比较了凯氏定氮法和杜马斯定氮法。除了比较Dumas方法的日常变异性之外，还评估了两个凯氏定氮系统和凯氏定氮算子之间的差异和变异性。凯氏定氮法给出的数值比杜马斯法略低，但明显更低。凯氏定氮法和杜马斯法都有相同的变异性（均值的标准差相同）。凯氏定氮法和杜马斯蛋白值的均值之比（K / D）在0.66至1.03之间。建立了转换方程y = -0.00536 + 0.97188x（R 2 = 0.9997）并验证了该转换方程可将杜马斯蛋白转换为凯氏定氮蛋白。

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  • 来源
    《Journal of the American Oil Chemists' Society》 |2003年第12期|1169-1173|共5页
  • 作者

    S. Jung; D. A. Rickert; N. A. Deak; E. D. Aldin; J. Recknor; L. A. Johnson; P. A. Murphy;

  • 作者单位

    Department of Food Science and Human Nutrition Iowa State UniversityCenter for Crops Utilization and Research Iowa State University;

    Department of Food Science and Human Nutrition Iowa State University;

    Department of Food Science and Human Nutrition Iowa State University;

    Department of Food Science and Human Nutrition Iowa State University;

    Department of Statistics Iowa State University;

    Department of Food Science and Human Nutrition Iowa State UniversityCenter for Crops Utilization and Research Iowa State University;

    Department of Food Science and Human Nutrition Iowa State UniversityCenter for Crops Utilization and Research Iowa State University;

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  • 正文语种 eng
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  • 关键词

    Dumas nitrogen; Kjeldahl nitrogen; protein; soybeans; soy protein;

    机译：杜马氮;凯氏氮;蛋白质;大豆;大豆蛋白质;

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