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首页> 外文期刊>Journal of Bioinformatics and Computational Biology >ANALYSIS OF qPCR DATA BY CONVERTING EXPONENTIALLY RELATED Ct VALUES INTO LINEARLY RELATED X0 VALUES
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ANALYSIS OF qPCR DATA BY CONVERTING EXPONENTIALLY RELATED Ct VALUES INTO LINEARLY RELATED X0 VALUES

机译：通过将指数相关的Ct值转换为线性相关的X0值来分析qPCR数据

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摘要

A common method for calculating results from qPCR experiments is the comparative Ct method, also called the 2-ΔΔCt method. However, several assumptions are included in the 2-ΔΔCt method and standard statistical analyses are not directly applicable. Here, we describe a different method, the X0 method, for result calculations and statistical analysis from qPCR experiments. The X0 method differs from the 2-ΔΔCt method by introducing a conversion of the exponentially related Ct values into linearly related X0 values, which represent the amount of starting material in a qPCR experiment. Results calculated by the X0 method are illustrated for qPCR experiments with technical and biological replicates, including procedures to calculate standard deviations. Incorporation of primer efficiencies in calculations by the X0 method is also described. Altogether, the X0 method constitutes a very simple and accurate alternative to the 2-ΔΔCt method for result calculations from qPCR data.
机译：qPCR实验计算结果的常用方法是比较Ct方法，也称为2-ΔΔCt方法。但是，2-ΔΔCt方法中包含一些假设，标准统计分析不能直接应用。在这里，我们描述了另一种方法，即X0方法，用于qPCR实验的结果计算和统计分析。 X0方法与2-ΔΔCt方法的不同之处在于，它引入了指数相关的Ct值到线性相关的X0值的转换，X0值表示qPCR实验中的起始物料量。通过X0方法计算得到的结果用于qPCR实验，包括技术和生物学重复，包括计算标准偏差的程序。还介绍了通过X0方法在计算中引入引物效率的方法。总而言之，X0方法构成了一种用于从qPCR数据进行结果计算的2-ΔΔCt方法的非常简单且准确的替代方法。

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    RUNE THOMSEN; CHRISTINA ANE ELISABETH SØLVSTEN; TOKE ELBEK LINNET; JENNY BLECHINGBERG; ANDERS LADE NIELSEN;

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    RUNE THOMSEN These authors contributed equally to the manuscript, shall be considered shared first authors.Department of Human Genetics, The Bartholin Buildin, Aarhus University, DK-8000 Aarhus C, DenmarkRune@humgen.au.dk CHRISTINA ANE ELISABETH SØLVSTEN These authors contributed equally to the manuscript, shall be considered shared first authors.Department of Human Genetics, The Bartholin Buildin, Aarhus University, DK-8000 Aarhus C, DenmarkChris@humgen.au.dk TOKE ELBEK LINNET Department of Human Genetics, The Bartholin Buildin, Aarhus University, DK-8000 Aarhus C, DenmarkToke@humgen.au.dk JENNY BLECHINGBERG Department of Human Genetics, The Bartholin Buildin, Aarhus University, DK-8000 Aarhus C, DenmarkJenny@humgen.au.dk ANDERS LADE NIELSEN Corresponding author.Department of Human Genetics, The Bartholin Buildin, Aarhus University, DK-8000 Aarhus C, Denmarkaln@humgen.au.dk;

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  • 关键词

    qPCR; replicates; ratio calculation; exponential; gene expression: ddCt method.;

    机译：定量PCR;复制品比率计算;指数基因表达：ddCt法。;

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