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首页> 外文期刊>Journal of Chinese Pharmaceutical Sciences >RP-HPLC Determination of Pinoresinol Diglucopyranoside in Qing'e Pill Extract
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RP-HPLC Determination of Pinoresinol Diglucopyranoside in Qing'e Pill Extract

机译：RP-HPLC法测定清e丸提取物中松脂醇二葡萄糖吡喃糖苷的含量

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摘要

Aim To develop and determine pinoresinol diglucopyranoside in Qing'e Pill, a traditional Chinese compound preparation containing Eucommia ulmoides Oliv. as the principal drug, by a reverse-phase high-performance liquid chromatographic method (RP-HPLC) . Methods The extract of Qing'e Pill was refluxed with 75% ethanol, purified on an AB-8 macroporous adsorption resin column and then injected into HPLC system. The HPLC assay was performed on an ODS analytical column with a mixture of methanol-acetonitrile-water (24:3:78, V/V/V) as the mobile phase at a flow-rate of 1.0 mL·min~(-1), and a UV detector set at 227 nm. Results Good linearity between peak area and concentration was found in the range of 5.5 - 170 μg·mL~(-1) for pinoresinol diglucopyranoside ( r > 0.9998) . The average recovery was 99.3%. The intra-day assay RSD and the inter-day assay RSD were 1.3% and 2.8%, respectively (n = 5). The content of pinoresinol diglucopyranoside in Qing'e Pill was determined to be 0.446 +- 0.012 mg·g~(-1) (n = 10). Conclusion The RP-HPLC method was proved to be sensitive, specific, accurate and precise for the determination of pinoresinol diglucopyranoside in Qing' e Pill.
机译：目的开发和测定青果丸中的松脂醇二葡萄糖吡喃糖苷，青果丸是含有杜仲杜仲的中药复方制剂。作为主要药物，通过反相高效液相色谱法（RP-HPLC）。方法将清e丸提取物与75％乙醇回流，在AB-8大孔吸附树脂柱上纯化后，注入HPLC系统。 HPLC测定在ODS分析柱上，以甲醇-乙腈-水（24：3：78，V / V / V）的混合物为流动相，流速为1.0 mL·min〜（-1 ）和设置为227 nm的紫外线检测器。结果松脂醇二葡萄糖吡喃葡萄糖苷的峰面积与浓度之间在5.5-170μg·mL〜（-1）范围内具有良好的线性关系（r> 0.9998）。平均回收率为99.3％。日内分析RSD和日间分析RSD分别为1.3％和2.8％（n = 5）。经测定，清e丸中松脂醇二葡萄糖吡喃糖苷的含量为0.446±0.012 mg·g〜（-1）（n = 10）。结论RP-HPLC法测定青娥丸中松脂醇二葡萄糖吡喃糖苷具有灵敏，特异，准确，准确的特点。

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