• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Computational Neuroscience >What we talk about when we talk about capacitance measured with the voltage-clamp step method
【24h】

What we talk about when we talk about capacitance measured with the voltage-clamp step method

机译：当我们谈论使用电压钳阶跃法测量的电容时所谈论的

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Capacitance is a fundamental neuronal property. One common way to measure capacitance is to deliver a small voltage-clamp step that is long enough for the clamp current to come to steady state, and then to divide the integrated transient charge by the voltage-clamp step size. In an isopotential neuron, this method is known to measure the total cell capacitance. However, in a cell that is not isopotential, this measures only a fraction of the total capacitance. This has generally been thought of as measuring the capacitance of the "well-clamped" part of the membrane, but the exact meaning of this has been unclear. Here, we show that the capacitance measured in this way is a weighted sum of the total capacitance, where the weight for a given small patch of membrane is determined by the voltage deflection at that patch, as a fraction of the voltage-clamp step size. This quantifies precisely what it means to measure the capacitance of the "well-clamped" part of the neuron. Furthermore, it reveals that the voltage-clamp step method measures a well-defined quantity, one that may be more useful than the total cell capacitance for normalizing conductances measured in voltage-clamp in nonisopotential cells.
机译：电容是一种基本的神经元特性。一种测量电容的常用方法是提供一个小的电压钳位阶跃，该步长足以使钳位电流达到稳态，然后将积分的瞬态电荷除以电压钳位阶跃大小。在等势神经元中，已知该方法可测量总细胞电容。但是，在非等电位单元中，这仅占总电容的一小部分。通常认为这是测量膜的“良好钳位”部分的电容，但是其确切含义尚不清楚。在这里，我们表明以这种方式测量的电容是总电容的加权总和，其中给定小片膜片的重量由该片片上的电压偏转决定，即电压钳位步长的一部分。这精确地量化了测量神经元“良好钳位”部分的电容的含义。此外，它揭示了电压钳位方法测量的是一个明确定义的数量，该数量可能比总电池电容更有用，用于归一化非等电位电池中电压钳位中测得的电导。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号