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首页> 外文期刊>Journal of Computational Neuroscience >Inferring connection proximity in networks of electrically coupled cells by subthreshold frequency response analysis
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Inferring connection proximity in networks of electrically coupled cells by subthreshold frequency response analysis

机译：通过亚阈值频率响应分析推断电耦合单元网络中的连接接近度

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Electrical synapses continuously transfer signals bi-directionally from one cell to another, directly or indirectly via intermediate cells. Electrical synapses are common in many brain structures such as the inferior olive, the subcoeruleus nucleus and the neocortex, between neurons and between glial cells. In the cortex, interneurons have been shown to be electrically coupled and proposed to participate in large, continuous cortical syncytia, as opposed to smaller spatial domains of electrically coupled cells. However, to explore the significance of these findings it is imperative to map the electrical synaptic microcircuits, in analogy with in vitro studies on monosynaptic and disynaptic chemical coupling. Since "walking" from cell to cell over large distances with a glass pipette is challenging, microinjection of (fluorescent) dyes diffusing through gapjunctions remains so far the only method available to decipher such microcircuits even though technical limitations exist. Based on circuit theory, we derive analytical descriptions of the AC electrical coupling in networks of isopotential cells. We then suggest an operative electro-physiological protocol to distinguish between direct electrical connections and connections involving one or more intermediate cells. This method allows inferring the number of intermediate cells, generalizing the conventional coupling coefficient, which provides limited information. We validate our method through computer simulations, theoretical and numerical methods and electrophysiological paired recordings.
机译：电突触通过中间细胞直接或间接地将信号从一个细胞双向双向传输到另一个细胞。电突触在神经元之间和神经胶质细胞之间的许多脑部结构中很常见，例如下橄榄，下小核层核和新皮层。在皮层中，中间神经元已经显示出是电耦合的，并提议参与大而连续的皮质合胞体，这与电耦合细胞的较小空间域相反。然而，要探索这些发现的重要性，必须像对单突触和双突触化学偶联的体外研究一样，绘制突触电微电路图。由于用玻璃移液管从细胞到细胞的“长距离行走”具有挑战性，因此，即使存在技术上的局限性，显微注射通过间隙连接扩散的（荧光）染料仍然是唯一可用来解密这种微电路的方法。基于电路理论，我们得出了等电位电池网络中交流电耦合的分析描述。然后，我们提出了一种有效的电生理方案，以区分直接电连接和涉及一个或多个中间细胞的连接。该方法可以推断出中间单元的数量，从而推广了常规的耦合系数，从而提供了有限的信息。我们通过计算机模拟，理论和数值方法以及电生理配对记录来验证我们的方法。

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