Application of triplex-PCR with an innovative combination of 3 pairs of primers for the detection of milk's animal origin in cheese and yoghurt

Evanthia Tsirigoti; Zoi Katsirma; Athanasios I. Papadopoulos; Georgios Samouris; Loukia V. Ekateriniadou; Evridiki Boukouvala

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Application of triplex-PCR with an innovative combination of 3 pairs of primers for the detection of milk's animal origin in cheese and yoghurt

机译：三重PC对3对引物的创新组合的应用在奶酪和酸奶中检测牛奶血管源的检测

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In this research communication we describe an innovative protocol that combines three pairs of primers, two from the literature and one designed in our laboratory, for application in triplex-PCR on somatic cell DNA to enable identification of the species origin (cow, sheep, goat) of cheeses and yogurts with a detection limit of 0.1%. Mislabeling was detected in 15 out of 40 cheeses and in 18 out of 40 yogurts tested. The suggested procedure is a quick and reliable tool for identifying the animal origin of cheeses and yogurts and it can be used to certify product reliability on the domestic and international market. Additionally, in combination with a serological test it can offer a reliable tool for detecting the presence of cow's whey.

机译：在这项研究中，我们描述了一种创新的协议，将三对引物组合在一起，从文献中的两双和在我们的实验室中设计，用于在体细胞DNA上的Triple-PCR应用，以实现物种来源（牛，羊，山羊）奶酪和酸奶的检出限为0.1％。在40个奶酪中有15个，在40个酸奶中检测到误标记。建议的程序是一种快速可靠的工具，用于识别奶酪和酸奶的动物来源，可用于在国内和国际市场上证明产品可靠性。此外，结合血清学测试，它可以提供可靠的工具，用于检测牛奶乳清的存在。

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来源
《Journal of dairy research》 |2020年第2期|239-242|共4页
作者
Evanthia Tsirigoti; Zoi Katsirma; Athanasios I. Papadopoulos; Georgios Samouris; Loukia V. Ekateriniadou; Evridiki Boukouvala;
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作者单位

Hellenic Agricultural Organization DEMETER Veterinary Research Institute Thessaloniki Greece Laboratory of Animal Physiology Sector of Zoology Department of Biology School of Sciences Aristotle University of Thessaloniki Thessaloniki Greece;

Hellenic Agricultural Organization DEMETER Veterinary Research Institute Thessaloniki Greece Laboratory of Animal Physiology Sector of Zoology Department of Biology School of Sciences Aristotle University of Thessaloniki Thessaloniki Greece;

Laboratory of Animal Physiology Sector of Zoology Department of Biology School of Sciences Aristotle University of Thessaloniki Thessaloniki Greece;

Hellenic Agricultural Organization DEMETER Veterinary Research Institute Thessaloniki Greece;

Hellenic Agricultural Organization DEMETER Veterinary Research Institute Thessaloniki Greece;

Hellenic Agricultural Organization DEMETER Veterinary Research Institute Thessaloniki Greece;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
Cheese; milk origin; triplex-PCR; yoghurt;

机译：起司;牛奶源;Triple-PCR;酸奶;

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专利

1. Developing a time and effort-effective, highly sensitive TaqMan probe-based real-time polymerase chain reaction protocol for the detection of Salmonella in milk, yoghurt, and cheese [J] . El-Sharoud Walid M. International Dairy Journal . 2015,第Null期

机译：开发省时省力，高灵敏度的基于TaqMan探针的实时聚合酶链反应方案，用于检测牛奶，酸奶和奶酪中的沙门氏菌
2. Application of a microwave-assisted extraction method for the extraction of organic acids from Greek cheeses and sheep milk yoghurt and subsequent analysis by ion-exclusion liquid chromatography. [J] . Papadakis E N, Polychroniadou A International Dairy Journal . 2005,第2期

机译：微波辅助提取法从希腊奶酪和羊奶酸奶中提取有机酸的应用，然后通过离子排斥液相色谱法进行分析。
3. Application of a microwave-assisted extraction method for the extraction of organic acids from Greek cheeses and sheep milk yoghurt and subsequent analysis by ion-exclusion liquid chromatography. [J] . Papadakis E N, Polychroniadou A International Dairy Journal . 2005,第2期

机译：微波辅助提取法从希腊奶酪和羊奶酸奶中提取有机酸的应用，然后通过离子排斥液相色谱法进行分析。
4. Improving the texture of cheese product from cow's milk through the coagulation process using a combination of papain and transglutaminase enzyme [C] . Adeboi F. Sheryl, Setiadi International Tropical Renewable Energy Conference . 2020

机译：使用木瓜和转谷氨酰胺酶的组合，通过凝血过程改善牛奶的奶酪产品质地
5. Development of a multiplex PCR for detection of Staphylococcus aureus in skim milk and cheddar cheese. [D] . Tamarapu, Sudhir. 2000

机译：开发用于检测脱脂牛奶和切达干酪中金黄色葡萄球菌的多重PCR。
6. Fermentation of Milk into Yoghurt and Cheese Leads to Contrasting Lipid and Glyceride Profiles [O] . Samuel Furse, Alexandre G. Torres, Albert Koulman 2019

机译：牛奶发酵成酸奶和奶酪会导致脂质和甘油酯的轮廓不同
7. Application of triplex-PCR with an innovative combination of 3 pairs of primers for the detection of milk's animal origin in cheese and yoghurt [O] . Evanthia Tsirigoti, Zoi Katsirma, Athanasios I. Papadopoulos, 2020

机译：三重PC对3对引物的创新组合的应用在奶酪和酸奶中检测牛奶血管源的检测

Application of triplex-PCR with an innovative combination of 3 pairs of primers for the detection of milk's animal origin in cheese and yoghurt

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