• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of dairy research >Characterization of biofilms and antimicrobial resistance of coagulase-negative Staphylococcus species involved with subclinical mastitis
【24h】

Characterization of biofilms and antimicrobial resistance of coagulase-negative Staphylococcus species involved with subclinical mastitis

机译：亚临床乳腺炎凝固酶阴性葡萄球菌物种的生物膜和抗微生物抗性的表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Biofilm formation is a central feature to guarantee staphylococcal persistence in hosts and is associated with several diseases that are difficult to treat. In this research paper, biofilm formation and antimicrobial susceptibility were investigated in staphylococcal strains belonging to several species. These strains were isolated from the milk of cows with subclinical mastitis and most of them were coagulase-negative, with the prevalence of Staphylococcus chromogenes. High genetic diversity was observed among the strains by pulsed field gel elec-trophoresis. Antimicrobial resistance was assessed by disk diffusion and more than 50% of the strains were resistant to ampicillin and penicillin G, with multi-resistance profiles (13.6%) also being observed. Most strains (65.9%) formed biofilms when cultivated in BHI supplemented with 1% glucose. Most strains (72.7%) carried the intercellular adhesion gene (icaA), while less than half (36.3%) carried the biofilm-associated protein gene (bap). Concentrations of up to 10xMIC of erythromycin and tetracycline were not sufficient to suppress cell viability in preformed biofilms. Our results revealed that a genetically diverse group of biofilm-forming Staphylococcus species can be involved in subclinical mastitis. Since high antimicrobial concentrations cannot eradicate biofilm cells in vitro, their use in dairy animals may be ineffective in controlling infections, while supporting selection of resistant microorganisms. These data reinforce the need for alternative therapies aiming at disrupting biofilms for effective disease control.
机译：生物膜形成是一种保证宿主中的葡萄球菌持久性的中心特征，并且与几种难以治疗的疾病有关。在本研究论文中，研究了属于几种物种的葡萄球菌菌株中的生物膜形成和抗微生物敏感性。这些菌株与亚临床乳腺炎的奶牛牛奶中分离出来，其中大部分是凝血酶阴性，具有葡萄球菌色度的患病率。脉冲场凝胶电流蛋白在菌株中观察到高遗传多样性。通过盘扩散评估抗微生物抗性，并且超过50％的菌株对氨苄青霉素和青霉素G具有抗性，并且也被观察到多重电阻曲线（13.6％）。在补充有1％葡萄糖的BHI培养时，大多数菌株（65.9％）形成生物膜。大多数菌株（72.7％）携带细胞间粘附基因（ICAA），而少于一半（36.3％）携带生物膜相关蛋白基因（BAP）。高达10×10xMic的红霉素和四环素的浓度不足以抑制在预制件生物膜中的细胞活力。我们的研究结果表明，遗传多样的生物膜形成葡萄球菌物种可参与亚临床乳腺炎。由于高抗微生物浓度不能在体外根除生物膜细胞，因此它们在乳制品中的使用可能导致控制感染，同时支持抗性微生物的选择。这些数据强化了需要替代疗法，旨在破坏生物膜以进行有效疾病控制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号