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首页> 外文期刊>Journal of dairy research >The effects of denatured major bovine whey proteins on the digestive tract, assessed by Caco-2 cell differentiation and on viability of suckling mice
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The effects of denatured major bovine whey proteins on the digestive tract, assessed by Caco-2 cell differentiation and on viability of suckling mice

机译：变性主要牛乳清蛋白对消化道的影响，由Caco-2细胞分化评估和乳酪生存性

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摘要

Alpha-lactalbumin (a-LA) and p-lactoglobulin (fS-LG) are contained in bovine milk whey. Chemical and physical treatments are known to alter the conformation of these proteins and we have previously reported that a-LA denatured with trifiuoroethanol (TFE) and isolated from sterilized market milk inhibited the growth of rat crypt IEC-6 cells. In the present study, we aimed to evaluate the effects of TFE-treated a-LA and β-LG on cell growth using cultured intestinal cells and on their safety using a suckling mouse model. First, we investigated the effect of the TFE-treated whey proteins on human colonic Caco-2 cells at various differentiation stages. In the undifferenti-ated stage, we assessed cell growth by a water-soluble tetrazolium-1 method. The native whey proteins enhanced cell proliferation, whereas the TFE-treated whey proteins strongly inhibited cell growth. We investigated cell barrier function in the post-differentiated stage by measuring transe-pithelial electrical resistance (TER). Not only native but also the TFE-treated whey proteins increased TER. Next, we evaluated whether the TFE-treated a-LA and β-LG have adverse effects on healthy suckling mice. No mice given by the TFE-treated samples showed any adverse symptoms. We also performed a safety test using a human rotavirus infected gastrointestinal disease suckling mice model. Even the TFE-treated whey proteins appeared to prevent the development of diarrheal symptoms without any adverse effects. Although we cannot know the effect of long-term ingestion of denatured whey proteins, these results suggest that they have no adverse effects on differentiated intestinal cells and digestive tract, at least in short-term ingestion.
机译：α-乳白蛋白（A-LA）和p-乳酰叶蛋白（FS-LG）含有牛奶乳清。已知化学和物理治疗改变这些蛋白质的构象，并且先前据报道，用三氟乙醇（TFE）变性的A-LA与灭菌的市场乳中分离的A-LA抑制了大鼠Crypt IEC-6细胞的生长。在本研究中，我们旨在评估TFE处理的A-LA和β-LG对培养的肠道细胞和使用乳胶小鼠模型的安全性的影响。首先，我们研究了TFE处理的乳清蛋白在各种分化阶段的人结肠Caco-2细胞上的作用。在未分子的阶段，我们通过水溶性四唑鎓-1方法评估细胞生长。本地乳清蛋白增强细胞增殖，而TFE处理的乳清蛋白强烈抑制细胞生长。通过测量Transe-Pithelial电阻（TER），我们研究了在分化后阶段的细胞屏障功能。不仅本土，而且还有TFE治疗的乳清蛋白增加了三倍。接下来，我们评估了TFE处理的A-LA和β-LG对健康哺乳小鼠是否具有不利影响。 TFE处理的样品没有给出的小鼠显示出任何不良症状。我们还使用人类轮状病毒感染的胃肠疾病哺乳小鼠模型进行了安全测试。即使是TFE治疗的乳清蛋白似乎均可预防腹泻症状的发展，而不会产生任何不利影响。虽然我们无法了解长期摄取变性乳清蛋白的影响，但这些结果表明它们对分化的肠细胞和消化道没有不利影响，至少在短期摄取中。

著录项

  • 来源
    《Journal of dairy research》 |2021年第2期|221-225|共5页
  • 作者

    Chihiro Kobayashi; Mizuho Inagaki; Midori Nohara; Mayuko Fukuoka; Xijier; Tomio Yabe; Yoshihiro Kanamarua;

  • 作者单位

    Faculty of Applied Biological Sciences Gifu University 1-1 Yanagido Gifu Gifu 501-1193 Japan;

    Faculty of Applied Biological Sciences Gifu University 1-1 Yanagido Gifu Gifu 501-1193 Japan;

    Faculty of Applied Biological Sciences Gifu University 1-1 Yanagido Gifu Gifu 501-1193 Japan;

    Faculty of Applied Biological Sciences Gifu University 1-1 Yanagido Gifu Gifu 501-1193 Japan;

    Faculty of Applied Biological Sciences Gifu University 1-1 Yanagido Gifu Gifu 501-1193 Japan;

    Faculty of Applied Biological Sciences Gifu University 1-1 Yanagido Gifu Gifu 501-1193 Japan Center for Highly Advanced Integration of Nano and Life Sciences (G-CHAIN) Gifu University 1-1 Yanagido Gifu Gifu 501-1193 Japan;

    Faculty of Applied Biological Sciences Gifu University 1-1 Yanagido Gifu Gifu 501-1193 Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    α-Lactalbumin; β-lactoglobulin; cell differentiation; cytotoxicity; denatured protein;

    机译：α-乳白酶;β-乳酰叶蛋白;细胞分化;细胞毒性;变性蛋白质;

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