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首页> 外文期刊>Journal of dairy research >Intracellular Staphylococcus aureus inhibits autophagy of bovine mammary epithelial cells through activating p38α
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Intracellular Staphylococcus aureus inhibits autophagy of bovine mammary epithelial cells through activating p38α

机译：细胞内金黄色葡萄球菌通过激活p38α抑制牛乳腺上皮细胞的自噬

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摘要

Staphylococcus aureus is a common pathogen of bovine mastitis which can induce autophagy and inhibit autophagy flux, resulting in intracellular survival and persistent infection. The aim of the current study was to investigate the role of p38α in the autophagy induced by intracellular S. aureus in bovine mammary epithelial cells. An intracellular infection model of MAC-T cells was constructed, and activation of p38α was examined after S. aureus invasion. Through activating/inhibiting p38α by anisomycin/SB203580, the autophagosomes, LC3 and p62 level were analyzed by immunofluorescence and western blot. To further study the detailed mechanism of p38α, phosphorylation of ULKlser757 was also detected. The results showed that intracellular S. aureus activated p38α, and the activation developed in a time-dependent manner. Inhibition of p38α promoted intracellular S. aurews-induced autophagy flow, up-regulated the ratio of LC3 Ⅱ/Ⅰ, reduced the level of p62 and inhibited the phosphorylation of ULKlser757, whereas the above results were reversed after activation of p38α. The current study indicated that intracellular S. aureus can inhibit autophagy flow by activating p38α in bovine mammary epithelial cells.
机译：金黄色葡萄球菌是牛乳腺炎的常见病原体，其可以诱导自噬并抑制自噬助体，导致细胞内存活性和持续的感染。目前研究的目的是探讨p38α在牛乳腺上皮细胞中的细胞内S.金黄色葡萄球菌诱导的自噬中的作用。构建了MAC-T细胞的细胞内感染模型，并在S. aureus侵袭后检查了P38α的活化。通过通过免疫荧光和Western印迹分析自噬霉素/ SB203580的激活/抑制p38α，通过免疫荧光和Western印迹分析自噬体，LC3和P62水平。为了进一步研究P38α的详细机制，还检测到ULKLSER757的磷酸化。结果表明，细胞内的金黄色葡萄球菌活化P38α，并以时间依赖的方式开发的激活。 P38α的抑制促进了细胞内S. AUREWS诱导的自噬流动，上调LC3Ⅱ/Ⅰ的比例降低了P62的水平并抑制ULKLSER757的磷酸化，而在P38α的激活后，上述结果逆转。目前的研究表明，通过在牛乳腺上皮细胞中激活P38α，细胞内的金黄色葡萄球菌可以抑制自噬流。

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  • 来源
    《Journal of dairy research》 |2021年第3期|293-301|共9页
  • 作者

    Run Wang; Wen Zhang; Lumei Wang; Na Geng; Xiaozhou Wang; Meihua Zhang; Jianzhu Liu; Yongxia Liu; Bo Han;

  • 作者单位

    College of Veterinary Medicine Shandong Agricultural University Tai'an Shandong China;

    College of Veterinary Medicine Shandong Agricultural University Tai'an Shandong China;

    College of Veterinary Medicine Shandong Agricultural University Tai'an Shandong China;

    College of Veterinary Medicine Shandong Agricultural University Tai'an Shandong China;

    College of Veterinary Medicine Shandong Agricultural University Tai'an Shandong China;

    College of Veterinary Medicine Shandong Agricultural University Tai'an Shandong China;

    Research Center for Animal Disease Control Engineering Shandong Agricultural University Tai'an Shandong China;

    College of Veterinary Medicine Shandong Agricultural University Tai'an Shandong China;

    College of Veterinary Medicine China Agricultural University Beijing China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Autophagy; bovine mammary epithelial cells; mastitis; p38α; Staphylococcus aureus;

    机译：自噬;牛乳腺上皮细胞;乳腺炎;P38α;葡萄球菌金黄色葡萄球菌;

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