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首页> 外文期刊>Journal of dairy research >Neutrophil and CD4~+ milk cell count related to natural incidence of mastitis in Jersey cattle
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Neutrophil and CD4~+ milk cell count related to natural incidence of mastitis in Jersey cattle

机译：中性粒细胞和CD4〜+乳细胞计数与泽西牛乳腺炎的自然发病率有关

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This Research Communication describes the relation between somatic cells and microbial content in milk from Jersey cattle. Milk samples were classified in groups: healthy, dirty and mastitic (from Staphylococcus spp., Escherichia coli, Coliforms). The somatic cells in each of those groups were analysed by two methods - flow cytometric and automatic fluorescent cell counting. Those methods were compared. Total somatic cell count (SCC), neutrophil count, and lymphocytes with cluster of differentiation 4 (CD4~+cells) were determined. There was a positive relationship between microbes and somatic cells. It was noticed that the neutrophil count was generally increased together with SCC, whilst the CD4~+ cell count was higher in healthy milk samples (about 8%) compared to mastitic ones (about 3%). Lower number of CD4~+ cells (from 1 to 4%) was determined in samples positive for Staphylococcus spp. but with lower SCC (from 2.7 to 4.0 × 10~5 cells/ml). Also, the number of CD4~+ cells in Staphylococcus spp.-positive samples increased (to 4.8%) together with higher SCC, something that was not observed in the other mastitic samples. Knowledge of those relations could be useful for veterinary medical tests in the initial phase of inflammation.
机译：该研究通信描述了泽西牛牛奶中体细胞与微生物含量之间的关系。牛奶样品分类为组：健康，脏和薄盐（来自葡萄球菌，大肠杆菌，大肠杆菌）。通过两种方法分析每个组中的体细胞 - 流式细胞术和自动荧光细胞计数。这些方法进行了比较。确定总体细胞计数（SCC），中性粒细胞计数和具有分化4（CD4〜+细胞）簇的淋巴细胞。微生物和体细胞之间存在正相关关系。注意到中性粒细胞计数通常与SCC一起增加，而与薄烟灰（约3％）相比，健康的牛奶样品（约8％）的CD4〜+细胞计数较高。在样品阳性的样品中，测定含量较低的CD4〜+细胞（从1-4％）。但是，低SCC（从2.7到4.0×10〜5个细胞/ ml）。此外，葡萄球菌SPP中的CD4〜+细胞的数量与较高的SCC一起增加（至4.8％），在其他悬霉样品中未观察到的东西。对这些关系的知识对于炎症初始阶段的兽医医学测试可能有用。

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