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首页> 外文期刊>Journal of dairy science >Technical note: Infusion, sampling, and vacuum-assisted collection devices for use in ruminally cannulated calves
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Technical note: Infusion, sampling, and vacuum-assisted collection devices for use in ruminally cannulated calves

机译：技术说明：用于瘤胃插管小牛的输液，取样和真空辅助收集装置

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Calves can be ruminally cannulated at young ages, but equipment size limitations preclude use of an infusion and sampling device in these small animals. Likewise, a procedure to easily evacuate rumen contents in young calves has not been described. Overcoming these technical complications related to assessment of ruminal passage kinetics, nutrient digestion, and volatile fatty acid absorption would aid in future studies advancing our knowledge of dairy calf nutrition. The first objective was to design and fabricate 2 devices (one device for infusion and sampling, and another for vacuum-assisted collection) suitable for use in young ruminally cannulated dairy calves. The second objective was to test the utility of these tools when performing procedures commonly used in ruminant nutrition research. A single weaned 62-d-old ruminally cannulated calf was used to evaluate the ability to infuse a solution of LiCoEDTA and sample rumen contents through the cannula cap over a period of 2 h to assess the rumen liquid passage rate (procedure 1). The device was capable of infusing the LiCoEDTA and sampling the rumen fluid, as evidenced by the presence of elevated Co concentrations in the sampled rumen fluid. Using the fluid samples obtained, liquid passage rate within the calf was estimated to be 40.2% of ruminal fluid/h. The second procedure tested the vacuum-assisted collection device and consisted of evacuating and weighing the rumen contents, which is considered a key preparatory step in washed reticulorumen technique experiments that aim to measure nutrient absorption. In agreement with existing literature, evacuated rumen contents represented approximately 4% of the calf's body weight. In conclusion, custom-built devices for infusion, sampling, and vacuum-assisted collection were efficacious when tested in a 62-d-old ruminally cannulated calf fed a diet of 100% texturized starter (18% crude protein, as-fed). Fellow scientists may employ and further modify these techniques to suit their needs when assessing passage kinetics, nutrient digestion, and volatile fatty acid absorption in calves.
机译：小牛可以在幼年时被瘤胃插管，但由于设备尺寸的限制，无法在这些小动物中使用输液和采样装置。同样，也没有描述容易排出幼小牛的瘤胃含量的方法。克服与评估瘤胃通过动力学，营养物质消化和挥发性脂肪酸吸收有关的这些技术复杂性，将有助于将来的研究，从而提高我们对小牛犊营养的认识。第一个目标是设计和制造2种设备（一种用于输液和采样的设备，另一种用于真空辅助收集的设备），适用于年轻的反刍动物插管奶牛犊。第二个目标是在执行反刍动物营养研究中常用的程序时测试这些工具的实用性。使用单只断奶的62 d大的瘤胃插管小牛来评估在2小时内通过套管帽注入LiCoEDTA溶液和样品瘤胃内含物的能力，以评估瘤胃液体通过率（过程1）。该设备能够注入LiCoEDTA并取样瘤胃液，这在取样的瘤胃液中存在高浓度的Co证明。使用获得的液体样品，小腿内的液体通过率估计为瘤胃液/ h的40.2％。第二步测试了真空辅助收集装置，包括抽空和称量瘤胃内容物，这被认为是洗涤网状技术实验中准备测量营养吸收的关键准备步骤。与现有文献一致，抽空的瘤胃含量约占小牛体重的4％。总而言之，当在62日龄的瘤胃插管小牛中饲喂100％膨化发酵剂（18％粗蛋白，进料）时，定制的用于输液，取样和真空辅助收集的装置是有效的。当评估犊牛的传代动力学，营养物质消化和挥发性脂肪酸吸收时，研究员可能会采用并进一步修改这些技术以适应他们的需求。

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