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首页> 外文期刊>Journal of dairy science >Microbial community structure and distribution in the air of a powdered infant formula factory based on cultivation and high-throughput sequence methods
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Microbial community structure and distribution in the air of a powdered infant formula factory based on cultivation and high-throughput sequence methods

机译：基于培养和高通量测序方法的婴儿奶粉工厂微生物群落结构和空气分布

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摘要

The air in a powdered infant formula (PIF) factory is a potential transfer medium for microorganisms. In this study, air samples from 6 main processing areas, almost covering the whole PIF processing line and 1 outdoor location, were collected from a PIF manufacturing plant during the winter and summer periods. A cultivation-based and an Illumina (San Diego, CA) high-throughput 16S rRNA sequencing method was used to investigate the community structures and distributions of bacteria in the air. High microbial diversity (25 genera, 56 species), with a dominant community including Staphylococcus, Bacillus, Acinetobacter, and Kocuria, was found by the cultivation-based method. Moreover, 104 genera were obtained from all samples by high-throughput sequencing methods. Lactococcus (32.3%), Bacillus (29.6%), and Staphylococcus (14.0%) were the preponderant genera. The indices from high-throughput sequencing results indicated that the bacterial community of the air samples was highly diverse. Significant differences in the diversity and distribution at 6 sampling locations were revealed using the 2 methods. In particular, the packaging process contained the highest proportion (39.4%) of isolated strains. The highest diversity in bacterial community structure was found in the outdoor location. More bacterial isolates and higher community diversity were observed in the summer samples compared with the winter samples. In addition, some pathogens, such as Acinetobacter baumannii, Bacillus cereus, and Staphylococcus cohnii, were mainly found in the large bag filling process, can filling, and packaging process areas. The present study provides greater insight into the microbial community and identifies potential sources of air contamination in PIF production environments and can serve as a guide to reduce the risk of microbial contamination in the production of PIF.
机译：婴儿配方奶粉（PIF）工厂中的空气是微生物的潜在转移介质。在这项研究中，在冬季和夏季，从一个PIF生产工厂收集了6个主要加工区域的空气样本，这些空气样本几乎覆盖了整个PIF生产线和1个室外位置。基于培养和Illumina（加利福尼亚州圣地亚哥）的高通量16S rRNA测序方法用于研究空气中细菌的群落结构和分布。通过基于栽培的方法发现了高微生物多样性（25属56种），并具有主要的群落，包括葡萄球菌，芽孢杆菌，不动杆菌和科库里亚。此外，通过高通量测序方法从所有样品中获得104属。乳球菌（32.3％），芽孢杆菌（29.6％）和葡萄球菌（14.0％）是主要的属。高通量测序结果的指标表明，空气样品的细菌群落高度多样。使用两种方法揭示了在6个采样位置的多样性和分布的显着差异。特别是，包装过程中分离菌株的比例最高（39.4％）。在室外位置发现细菌群落结构的多样性最高。与冬季样品相比，夏季样品中观察到更多细菌分离株和更高的群落多样性。另外，一些病原体，例如鲍曼不动杆菌，蜡状芽孢杆菌和cohnphylococcus cohnii，主要出现在大袋装，罐装和包装过程中。本研究提供了对微生物群落的更深入了解，并确定了PIF生产环境中潜在的空气污染源，并可作为降低PIF生产中微生物污染风险的指南。

著录项

  • 来源
    《Journal of dairy science》 |2018年第8期|6915-6926|共12页
  • 作者

    Wu Shuang; Jiang Yujun; Lou Binbin; Feng Jing; Zhou Yanhong; Guo Ling; Forsythe Stephen J.; Man Chaoxin;

  • 作者单位

    Northeast Agr Univ, Dept Food Sci, Minist Educ, Key Lab Dairy Sci, Harbin 150030, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Dept Food Sci, Minist Educ, Key Lab Dairy Sci, Harbin 150030, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Dept Food Sci, Minist Educ, Key Lab Dairy Sci, Harbin 150030, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Dept Food Sci, Minist Educ, Key Lab Dairy Sci, Harbin 150030, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Dept Food Sci, Minist Educ, Key Lab Dairy Sci, Harbin 150030, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Dept Food Sci, Minist Educ, Key Lab Dairy Sci, Harbin 150030, Heilongjiang, Peoples R China;

    Foodmicrobe Com, Nottingham NG12 5GY, England;

    Northeast Agr Univ, Dept Food Sci, Minist Educ, Key Lab Dairy Sci, Harbin 150030, Heilongjiang, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    airborne microorganism; bacterial diversity; powdered infant formula; milk; high-throughput sequencing;

    机译：空气传播微生物;细菌多样性;粉状婴儿配方食品;牛奶;高通量测序;

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