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首页> 外文期刊>Infection and immunity >Cross-reactive, cell-associated antigen on L929 cells infected with temperature-sensitive mutants of sindbis virus.
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Cross-reactive, cell-associated antigen on L929 cells infected with temperature-sensitive mutants of sindbis virus.

机译：L929细胞上的交叉反应性，细胞相关抗原感染SINDBIS病毒的温度敏感突变体。

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Temperature-sensitive (ts) mutants of Sindbis virus (SIN) were used to aid in the identification of alphavirus cross-reactive proteins on the surface of infected cells by antibody-dependent, complement-mediated cytolysis. Antisera prepared in rabbits against purified SIN or Semliki Forest viruses were highly cytotoxic for cells infected with wild-type SIN and for cells infected at the permissive temperature with maturation-defective, ts mutants of SIN belonging to several distinct complementation groups. When these SIN mutants were analyzed by antibody-dependent, complement-mediated cytolysis at the restrictive temperature only cells infected with the SIN mutant of complementation group E, ts20, participated in both homologous (with anti-SIN serum) and heterologous (with anti-Semliki Forest virus serum) antibody-dependent, complement-mediated cytolysis reactions. These data and the known defect of ts20 suggested that the cell-associated viral E1 glycoprotein was a functional target antigen for homologous and cross-immunoreactivity in alphavirus-infected cells. At the restrictive temperature there were quantitative differences in antibody-dependent, complement-mediated cytolysis reactivity of ts20- versus wild type-infected cells consistent with the suggestion that ts20-infected cells do not fully express all of the homologous or the cross-reactive antigenic determinants found in wild-type infection. Additional potential sites for antigenic determinants involved in alphavirus-immune cross-reactivity are discussed in relation to events in virus maturation.
机译：SINDBIS病毒（SIN）的温度敏感（TS）突变体用于通过抗体依赖性补体介导的细胞分解有助于鉴定感染细胞表面上的alphaVirus交叉反应蛋白。在纯化纯化的SIN或Semliki林病毒中含有抗血清的抗血清对于感染野生型SIN感染的细胞和在允许血液中感染的细胞，具有成熟缺陷，属于若干不同的互补组的突变体的细胞是高度细胞毒性。当通过依赖于抗体依赖性分析这些SIN突变体时，在限制性温度下介导的细胞溶解，仅在互补族互补群E，TS20的SIN突变体感染的细胞中参与同源（抗SIN血清）和异源（抗 - Semliki林病毒血清）抗体依赖性，补蛋白介导的细胞分解反应。这些数据和TS20的已知缺陷表明细胞相关病毒E1糖蛋白是用于在alphaVirous感染细胞中同源和交叉免疫反应性的功能靶抗原。在限制温度下，抗体依赖性的抗体依赖性的定量差异，与TS20-与野生型细胞的细胞分解反应性一致，这与TS20感染细胞没有完全表达所有同源或交叉反应性抗原的建议在野生型感染中发现的决定因素。讨论了与病毒成熟中的事件讨论了参与alphaVirous-免疫交叉反应性的抗原决定因素的额外潜在部位。

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