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首页> 外文期刊>Journal of Environmental Protection and Ecology >HEMATOLOGICAL PARAMETERS AS INDICATORS OF TOXIC STRESS PRODUCED BY MYCOTOXIN FOOD CONTAMINATION IN THE EUROPEAN CATFISH (Silurus glanis L.)
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HEMATOLOGICAL PARAMETERS AS INDICATORS OF TOXIC STRESS PRODUCED BY MYCOTOXIN FOOD CONTAMINATION IN THE EUROPEAN CATFISH (Silurus glanis L.)

机译：血液学参数作为欧洲食肉中被霉菌毒素食物污染所产生的毒性应激的指标

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摘要

Hematological indices are important parameters for the evaluation of fish physiological status. The aim of the present study was to obtain a basic knowledge of the hematological response of the European catfish under the action of toxic metabolites (aflatoxin B) secreted by the mold Aspergillus flavus, but also the assessment of the physiological changes. The sampling of catfish blood from the two variants (infected and uninfected fish) before and after the experimental trial allowed the determination of the hematological indices. The erythrocyte count (EC) was done in an improved Neubauer haemocytometer, using the Vulpian reagent as dilution, following the method of Svobodova. The microhaematocrit method of Blaxhall and Darsley was employed in the determination of blood haematocrit, assessed through the centrifugation of capillaries to 12 000 rpm. The haemoglobin content of blood samples was determined by the Sahli hemoglobinmeter method. The hematological indices: mean cell volume (MCV), mean cell hemoglobin (MCH) and mean cell hemoglobin concentration (MCHC) were calculated using the formula of Svobodova. In the infected fish with aflatoxin, a decrease of RBC (-16.24%), PVC (-6%), Hb (-18.2%), MCH (-2.12%) and MCHC (-13.3%) and an increase of MCV (+11.5%) was observed, compared to uninfected fish. The decrease of the number of erythrocytes, the amount of haemoglobin and haematocrit in the blood of infected fish led to the installation of a state of anemia.
机译：血液学指标是评价鱼类生理状况的重要参数。本研究的目的是获得欧洲cat鱼在霉菌黄曲霉分泌的有毒代谢产物（黄曲霉毒素B）作用下的血液学反应的基础知识，以及对生理变化的评估。在试验之前和之后，从两种变体（感染和未感染的鱼）中采集of鱼血样可以确定血液学指标。按照Svobodova的方法，使用Vulpian试剂作为稀释液，在改良的Neubauer血细胞计数器中进行红细胞计数（EC）。 Blaxhall和Darsley的微血细胞比容法用于确定血液中的血细胞比容，通过将毛细管离心至12000 rpm进行评估。血样中的血红蛋白含量通过Sahli血红蛋白仪方法测定。血液学指标：平均细胞体积（MCV），平均细胞血红蛋白（MCH）和平均细胞血红蛋白浓度（MCHC）使用Svobodova公式计算。在感染了黄曲霉毒素的鱼中，RBC（-16.24％），PVC（-6％），Hb（-18.2％），MCH（-2.12％）和MCHC（-13.3％）下降，MCV（与未感染鱼相比，观察到+ 11.5％）。受感染鱼的血液中红细胞数量，血红蛋白和血细胞比容的减少导致了贫血的发生。

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  • 来源
    《Journal of Environmental Protection and Ecology》 |2011年第4期|p.1898-1903|共6页
  • 作者

    A. DOCAN; V. CRISTEA; L. DEDIU; I. GRECU;

  • 作者单位

    Faculty of Food Science and Engineering, Department of Aquaculture,Environment Science and Cadastre, University 'Dunarea de Jos 'of Galati,61-63 Garii Street, 800 003 Galati, Romania;

    Faculty of Food Science and Engineering, Department of Aquaculture,Environment Science and Cadastre, University 'Dunarea de Jos 'of Galati,61-63 Garii Street, 800 003 Galati, Romania;

    Faculty of Food Science and Engineering, Department of Aquaculture,Environment Science and Cadastre, University 'Dunarea de Jos 'of Galati,61-63 Garii Street, 800 003 Galati, Romania;

    Faculty of Food Science and Engineering, Department of Aquaculture,Environment Science and Cadastre, University 'Dunarea de Jos 'of Galati,61-63 Garii Street, 800 003 Galati, Romania;

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  • 正文语种 eng
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  • 关键词

    silurus glanis; hematological indices; aflatoxin; anemia;

    机译：Silurus glanis;血液学指标黄曲霉毒素贫血;

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