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首页> 外文期刊>Journal of environmental science and health >Genotypic characterization of quinolone resistant-Escherichia coli isolates from retail food in Morocco
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Genotypic characterization of quinolone resistant-Escherichia coli isolates from retail food in Morocco

机译：摩洛哥零售食品对喹诺酮类耐药大肠杆菌分离株的基因型鉴定

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摘要

This study was conducted to assess the retail food as a possible vehicle for antimicrobial resistant, particularly quinolones resistant and pathogenic Escherichia coli. We determined the prevalence and characteristics of nalidixic acid (Nal) resistant £ coli isolates from diverse retail food samples. In all, 70 (28%) of 250 E. coli isolates studied were Nal-resistant E. coli and 91% of these were multi-drug resistant. Plasmid mediated quinolone resistance genes were identified in 32 isolates, including aac(6')-lb-cr (n = 16), qnrS1 (n = 11) and qnrB19 (n = 7). Mutations in gyr A and par C genes were detected among 80% of the isolates, and the isolates showed substitution Ser83-Leu and Asp87-Asn in gyrA and Ser80-lle in parC. In addition, three different gene cassettes were identified (aadA1, aadA7, aac(3)-ld) in 18%. Virulence-associated genes stx1, eae, sfa, hlyA and stx2 were found in six (8%), three (4%), two (3%), three (4%) and three (4%) isolates, respectively. E coli isolates of phylogenetic group A were dominant (64%, 45/70). Pulsed field gel electrophoresis revealed none epidemiological relationship between these isolates. The results of this work report the higher frequency of Nat-resistant E coli isolates from Moroccan retail food samples including MDR and pathogenic isolates.
机译：进行这项研究是为了评估零售食品是否具有抗药性，尤其是对喹诺酮类药物和致病性大肠杆菌的抗药性。我们确定了来自各种零售食品样本的耐萘啶酸（Nal）的大肠杆菌分离株的患病率和特征。总共研究的250株大肠杆菌中有70株（28％）是耐Nal的大肠杆菌，其中91％是多药耐药的。在32个分离株中鉴定了质粒介导的喹诺酮抗性基因，包括aac（6'）-lb-cr（n = 16），qnrS1（n = 11）和qnrB19（n = 7）。在80％的分离物中检测到gyr A和par C基因突变，这些分离物在gyrA中显示了Ser83-Leu和Asp87-Asn，在parC中显示了Ser80-lle。此外，鉴定出18％的三个不同基因盒（aadA1，aadA7，aac（3）-ld）。与毒力相关的基因stx1，eae，sfa，hlyA和stx2分别在六个（8％），三个（4％），两个（3％），三个（4％）和三个（4％）分离物中发现。系统发育组A的大肠杆菌分离物占主导（64％，45/70）。脉冲场凝胶电泳显示这些分离株之间没有流行病学关系。这项工作的结果表明，来自摩洛哥零售食品样本（包括MDR和致病菌）的耐Nt大肠杆菌分离株的频率更高。

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  • 来源
    《Journal of environmental science and health》 |2017年第3期|107-114|共8页
  • 作者

    Kaotar Nayme; Abouddihaj Barguigua; Brahim Bouchrif; Bouchra Karraouan; Fatima El Otmani; Naima Elmdaghri; Khalid Zerouali; Mohammed Timinouni;

  • 作者单位

    Molecular Bacteriology Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco,Microbiology Laboratory, Faculty of Medicine and Pharmacy, University Hassan II, Casablanca, Morocco;

    Faculty of Medicine and Pharmacy, University Sultan Moulay Slimane, Beni Mellal, Morocco;

    Food Microbiology Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco;

    Microbiology, Health and Environment Team Research, Department of Biology, Faculty of Sciences, ChouaibDoukkali University, El Jadida, Morocco;

    University Hospital Center Ibn Rochd, Casablanca, Morocco;

    Molecular Bacteriology Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco,Microbiology Laboratory, Faculty of Medicine and Pharmacy, University Hassan II, Casablanca, Morocco,University Hospital Center Ibn Rochd, Casablanca, Morocco;

    Microbiology Laboratory, Faculty of Medicine and Pharmacy, University Hassan II, Casablanca, Morocco,University Hospital Center Ibn Rochd, Casablanca, Morocco;

    Molecular Bacteriology Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco;

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  • 正文语种 eng
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  • 关键词

    E coli; Morocco; PFGE; quinolone-resistance; retail food; virulence genes;

    机译：大肠杆菌;摩洛哥PFGE;耐喹诺酮类;零售食品;毒力基因;

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