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首页> 外文期刊>Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering >Amperometric determination of cadmium, lead, and mercury metal ions using a novel polymer immobilised horseradish peroxidase biosensor system
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Amperometric determination of cadmium, lead, and mercury metal ions using a novel polymer immobilised horseradish peroxidase biosensor system

机译：使用新型固定化辣根过氧化物酶生物传感器系统的安培法测定镉，铅和汞金属离子

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This work was undertaken to develop a novel Pt/PANI-co-PDTDA/HRP biosensor system for environmental applications to investigate the inhibition studies by specific heavy metals, to provide data suitable for kinetic studies and further application of the biosensor to environmental samples. The newly constructed biosensor was compared to the data of the well-researched Pt/ PANI/HRP biosensor. Optimised experimental conditions, such as the working pH for the biosensor was evaluated. The functionality of the amperometric enzyme sensor system was demonstrated by measuring the oxidation current of hydrogen peroxide followed by the development of an assay for determination of metal concentration in the presence of selected metal ions of Cd~(2+), Pb~(2+) and Hg~(2+). The detection limits were found to be 8 × 10~(-4) μg L~(-1) for cadmium, 9.38 × 10~(-4) μg L~(-1) for lead and 7.89 × 10~(-4) μg L~(-1) for mercury. The World Health Organisation recommended that the maximum safety level of these metals should not exceed 0.005 mg L~(-1) of Cd~(2+), 0.01 mg L~(-1) of Pb~(2+) and 0.001 mg L~(-1) of Hg~(2+), respectively. The analytical and detection data for the metals investigated were observed to be lower than concentrations recommended by several bodies including World Health Organisation and Environmental Protection Agencies. Therefore the biosensors developed in this study can be used to screen the presence of these metals in water samples because of its low detection limit. The modes of inhibition of horseradish peroxidase by Pb~(2+), Cd~(2+) and Hg~(2+) as analysed using the double reciprocal plots of the Michaelis-Menten equation was found to be reversible and uncompetitive inhibition. Based on the K_m~(app) and I_(max) values for both biosensors the results have shown smaller values. These results also proved that the enzyme modified electrode is valuable and can be deployed for the determination or screening of heavy metals.
机译：开展这项工作是为了开发一种新型Pt / PANI-co-PDTDA / HRP生物传感器系统，用于环境应用，以研究特定重金属的抑制研究，提供适用于动力学研究的数据以及将该生物传感器进一步应用于环境样品的数据。将新构建的生物传感器与经过充分研究的Pt / PANI / HRP生物传感器的数据进行比较。评估了优化的实验条件，例如生物传感器的工作pH。通过测量过氧化氢的氧化电流，然后开发一种在选定的Cd〜（2 +），Pb〜（2+）金属离子存在下测定金属浓度的测定方法，证明了安培酶传感器系统的功能。 ）和Hg〜（2+）。镉的检出限为8×10〜（-4）μgL〜（-1），铅为9.38×10〜（-4）μgL〜（-1）和7.89×10〜（-4） ）μgL〜（-1）对于汞。世界卫生组织建议这些金属的最大安全水平不应超过Cd〜（2+）的0.005 mg L〜（-1），Pb〜（2+）的0.01 mg L〜（-1）和0.001 mg Hg〜（2+）的L〜（-1）。据观察，所调查金属的分析和检测数据低于包括世界卫生组织和环境保护机构在内的几个机构所建议的浓度。因此，本研究开发的生物传感器由于其检测限低，因此可用于筛选水样中这些金属的存在。使用Michaelis-Menten方程的双向倒数图分析了Pb〜（2 +），Cd〜（2+）和Hg〜（2+）对辣根过氧化物酶的抑制模式，是可逆的且竞争性的抑制。基于两个生物传感器的K_m〜（app）和I_（max）值，结果显示较小的值。这些结果还证明了酶修饰的电极是有价值的，并且可以用于确定或筛选重金属。

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