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首页> 外文期刊>Journal of Environmental Science and Health >Physiological effect of multi-walled carbon nanotubes (MWCNTs) on conidia of the entomopathogenic fungus, Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes)
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Physiological effect of multi-walled carbon nanotubes (MWCNTs) on conidia of the entomopathogenic fungus, Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes)

机译：多壁碳纳米管（MWCNTs）对昆虫病原真菌，烟曲拟青霉（Deuteromycotina：Hyphomycetes）分生孢子的生理效应

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The aim of the investigation was to assess the influence of commercial and carboxylated multi-walled carbon nanotubes (MWCNTs) on conidia of the entomopathogenic fungus Paecilomyces fumosoroseus. The commercial MWCNTs had an external diameter of about 40-60 nm, a length of 300-600 nm, a specific density of 140 to 300 g/dm~3 and a carbon content of above 80%. Carboxylated MWCNTs were obtained by oxidizing commercial MWCNT by heating in HNO_3, filtering, washing with water and drying. Conidia after different times of contact (from 1 to 865 hours) with nanomaterials in aqueous solutions were cultured for linear and biomass growth. Growth and sporification of mycelium after culture were evaluated. MWCNTs are not greatly cytotoxic for P. fumosoroseus conidia in the applied concentrations. The linear growth of mycelium obtained from conidia after contact with nanotubes was inhibited only in 2 (out of 18) cases (ones for both kinds of nanotubes). Carboxylated nanotubes did not inhibit biomass growth at all, but commercial nanotubes inhibited biomass growth in 2 cases. Sporification was the feature most strongly modified by carbon nanotubes. The commercial nanotubes again more strongly limited sporification of mycelium than carboxylated nanotubes did. The relatively greater influence of commercial versus carboxylated nanotubes was observed in the experiments.
机译：该研究的目的是评估商业和羧化多壁碳纳米管（MWCNT）对昆虫病原真菌fuesoroseus的分生孢子的影响。市售的MWCNT具有约40-60nm的外径，300-600nm的长度，140-300g / dm 3的比重和碳含量高于80％。通过在HNO 3中加热，氧化，过滤，用水洗涤并干燥来氧化商业化的MWCNT，从而获得羧化的MWCNT。与水溶液中的纳米材料接触不同时间（从1到865小时）后的分生孢子进行线性和生物量生长。培养后评估菌丝体的生长和孢子化。在所应用的浓度下，MWCNTs对于烟曲霉分生孢子的细胞毒性不是很大。与纳米管接触后，从分生孢子获得的菌丝体的线性生长仅在2种（18种）情况下受到抑制（两种纳米管均发生这种情况）。羧基化的纳米管根本没有抑制生物量的增长，但是商业化的纳米管在2例中抑制了生物量的增长。孢子化是碳纳米管最强烈地修饰的特征。与羧化纳米管相比，商业化的纳米管再次更强烈地限制了菌丝体的孢子形成。在实验中观察到商业化的相对于羧化的纳米管的相对较大的影响。

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