首页> 外文期刊>Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering >Laboratory Study on the Immobilization of Bacterial Spores in Arid Environments
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Laboratory Study on the Immobilization of Bacterial Spores in Arid Environments

机译:干旱环境中细菌芽孢固定化的室内研究

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In this study, two effective, non-toxic, wind erosion palliative materials were analyzed for their efficacy in preventing the spread of bacterial spores. Desert sand was employed in a laboratory setting with a non-toxic simulant bacterium in an attempt to accurately represent the spreadability of the hantavirus. Spore simulants were used instead of viruses due to availability, decreased susceptibility to desiccation and detection ability without involving tissue cultures. The simulant was used to contaminate sand in a controlled environment, and an artificial turbulence was introduced using compressed air to generate airflow that could be expected in a desert environment. The airborne spores were identified both qualitatively and quantitatively through microscopy, Gram staining, plating, and incubation to monitor effectiveness. A water-based polysaccha-ride product, Surtac, was found to be most effective for the immobilization of bacteria on sand and greatly reduced the amount of contaminant that becomes airborne. The results suggest that the two wind erosion products used in this study may be successfully employed to reduce the ability of bacterial spores to spread in arid regions.
机译:在这项研究中,分析了两种有效的无毒风蚀姑息治疗材料在预防细菌孢子扩散方面的功效。在实验室环境中,将沙漠沙与无毒的模拟细菌一起使用,以试图准确代表汉坦病毒的传播能力。由于可利用性,不涉及组织培养,降低了干燥敏感性和检测能力,因此使用孢子模拟物代替病毒。该模拟物用于在受控环境中污染沙子,并使用压缩空气引入了人工湍流,以产生在沙漠环境中可以预期的气流。通过显微镜,革兰氏染色,铺板和孵育以监测效果,从质量和数量上鉴定了空气中的孢子。发现一种水基多糖骑乘产品Surtac对于将细菌固定在沙子上最有效,并大大减少了空气中污染物的数量。结果表明,本研究中使用的两种风蚀产品可能已成功用于降低细菌孢子在干旱地区扩散的能力。

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