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首页> 外文期刊>Journal of Experimental Botany >Relationship between asparagine metabolism and protein concentration in soybean seed
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Relationship between asparagine metabolism and protein concentration in soybean seed

机译：大豆种子中天冬酰胺代谢与蛋白质浓度的关系

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摘要

The relationship between asparagine metabolism and protein concentration was investigated in soybean seed. Phenotyping of a population of recombinant inbred lines adapted to Illinois confirmed a positive correlation between free asparagine levels in developing seeds and protein concentration at maturity. Analysis of a second population of recombinant inbred lines adapted to Ontario associated the elevated free asparagine trait with two of four quantitative trait loci determining population variation for protein concentration, including a major one on chromosome 20 (linkage group I) which has been reported in multiple populations. In the seed coat, levels of asparagine synthetase were high at 50 mg and progressively declined until 150 mg seed weight, suggesting that nitrogenous assimilates are pre-conditioned at early developmental stages to enable a high concentration of asparagine in the embryo. The levels of asparaginase B1 showed an opposite pattern, being low at 50 mg and progressively increased until 150 mg, coinciding with an active phase of storage reserve accumulation. In a pair of genetically related cultivars, ∼2-fold higher levels of asparaginase B1 protein and activity in seed coat, were associated with high protein concentration, reflecting enhanced flux of nitrogen. Transcript expression analyses attributed this difference to a specific asparaginase gene, ASPGB1a. These results contribute to our understanding of the processes determining protein concentration in soybean seed.
机译：研究了大豆种子中天冬酰胺代谢与蛋白质浓度之间的关系。适应伊利诺伊州的重组自交系群体的表型确定了发育中的种子中游离天冬酰胺水平与成熟蛋白质浓度之间呈正相关。分析适用于安大略省的第二个重组自交系种群，将其升高的游离天冬酰胺特性与四个定量性状位点中的两个联系起来，以确定蛋白质浓度的群体变异，其中包括第20号染色体上的一个主要基因（I型连锁），已有报道人口。在种皮中，天冬酰胺合成酶的水平高达50 mg，然后逐渐下降，直到种子重量达到150 mg，这表明含氮的同化物在发育的早期就进行了预处理，以使胚胎中的天冬酰胺含量高。天冬酰胺酶B1的水平显示出相反的模式，低至50 mg，然后逐渐增加直至150 mg，这与储存储备积累的活跃期相吻合。在一对与遗传相关的品种中，高约2倍的天冬酰胺酶B1蛋白水平和种皮中的活性与高蛋白浓度相关，反映了氮通量的提高。转录表达分析将这种差异归因于特定的天冬酰胺酶基因ASPGB1a。这些结果有助于我们理解确定大豆种子中蛋白质浓度的过程。

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  • 来源
    《Journal of Experimental Botany》 |2012年第8期|p.3173-3184|共12页
  • 作者

    Sudhakar Pandurangan12 Agnieszka Pajak2 Stephen J. Molnar3 Elroy R. Cober3 Sangeeta Dhaubhadel12 Cinta Hernández-Sebastià2 Werner M. Kaiser4 Randall L. Nelson5 Steven C. Huber6* and Frédéric Marsolais12*;

  • 作者单位

    1Department of Biology, University of Western Ontario, London, Ontario, N6A 5B7, Canada 2Agriculture and Agri-Food Canada, Genomics and Biotechnology, Southern Crop Protection and Food Research Centre, 1391 Sandford St., London, Ontario, N5V 4T3, Canada 3Agriculture and Agri-Food Canada, Bioproducts and Bioprocesses and Sustainable Production Systems, Eastern Cereal and Oilseeds Research Centre, Central Experimental Farm, Ottawa, Ontario, K1A 0C6, Canada 4Department of Botany I, Julius-von-Sachs-Institute for Biosciences, University of Würzburg, D-97082 Würzburg, Germany 5US Department of Agriculture-Agricultural Research Service, Soybean/Maize Germplasm, Pathology, and Genetics Research Unit, Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA 6US Department of Agriculture-Ag;

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