...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >A bHLH transcription factor, DvIVS, is involved in regulation of anthocyanin synthesis in dahlia (Dahlia variabilis)
【24h】

A bHLH transcription factor, DvIVS, is involved in regulation of anthocyanin synthesis in dahlia (Dahlia variabilis)

机译：bHLH转录因子DvIVS参与大丽花（Dahlia variabilis）花色苷合成的调控

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Dahlias (Dahlia variabilis) exhibit a wide range of flower colours because of accumulation of anthocyanin and other flavonoids in their ray florets. Two lateral mutants were used that spontaneously occurred in ‘Michael J’ (MJW) which has yellow ray florets with orange variegation. MJOr, a bud mutant producing completely orange ray florets, accumulates anthocyanins, flavones, and butein, and MJY, another mutant producing completely yellow ray florets, accumulates flavones and butein. Reverse transcription–PCR analysis showed that expression of chalcone synthase 1 (DvCHS1), flavanone 3-hydroxylase (DvF3H), dihydroflavonol 4-reductase (DvDFR), anthocyanidin synthase (DvANS), and DvIVS encoding a basic helix–loop–helix transcription factor were suppressed, whereas that of chalcone isomerase (DvCHI) and DvCHS2, another CHS with 69% nucleotide identity with DvCHS1, was not suppressed in the yellow ray florets of MJY. A 5.4 kb CACTA superfamily transposable element, transposable element of Dahlia variabilis 1 (Tdv1), was found in the fourth intron of the DvIVS gene of MJW and MJY, and footprints of Tdv1 were detected in the variegated flowers of MJW. It is shown that only one type of DvIVS gene was expressed in MJOr, whereas these plants are likely to have three types of the DvIVS gene. On the basis of these results, the mechanism regulating the formation of orange and yellow ray florets in dahlia is discussed.
机译：大丽花（Dahlia variabilis）由于花色苷和其他类黄酮在射线小花中的积累而表现出多种花色。使用了两个侧向突变体，它们自发地出现在“ Michael J”（MJW）中，该突变体具有黄色射线的小花和橙色的杂色。产生完全橙色射线小花的芽突变体MJOr积累花青素，黄酮和丁烯，而产生完全黄色射线小花的另一突变体MJY积累黄酮和丁。逆转录-PCR分析显示查尔酮合酶1（DvCHS1），黄烷酮3-羟化酶（DvF3H），二氢黄酮醇4-还原酶（DvDFR），花色素苷合酶（DvANS）和编码基本螺旋-环-螺旋转录因子的DvIVS的表达被抑制，而查耳酮异构酶（DvCHI）和DvCHS2，与DvCHS1具有69％核苷酸同一性的另一个CHS，在MJY的黄色射线小花中没有被抑制。在MJW和MJY的DvIVS基因的第四个内含子中发现了一个5.4 kb的CACTA超家族转座因子，即大丽花变种1（Tdv1）的转座因子，并且在MJW杂色花中检测到Tdv1的足迹。已显示在MJOr中仅表达一种类型的DvIVS基因，而这些植物可能具有三种类型的DvIVS基因。根据这些结果，讨论了调节大丽花中橙色和黄色射线小花形成的机理。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号