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首页> 外文期刊>Journal of Experimental Botany >Low temperature and light regulate delta 12 fatty acid desaturases (FAD2) at a transcriptional level in cotton (Gossypium hirsutum)
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Low temperature and light regulate delta 12 fatty acid desaturases (FAD2) at a transcriptional level in cotton (Gossypium hirsutum)

机译：低温和光调节棉花（陆地棉）在转录水平上的δ12脂肪酸去饱和酶（FAD2）。

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摘要

Lipid modifying enzymes play a key role in the development of cold stress tolerance in cold-resistant plants such as cereals. However, little is known about the role of the endogenous enzymes in cold-sensitive species such as cotton. Delta 12 fatty acid desaturases (FAD2), known to participate in adaptation to low temperatures through acyl chain modifications were used in gene expression studies in order to identify parameters of plant response to low temperatures. The induction of microsomal delta 12 fatty acid desaturases at an mRNA level under cold stress in plants is shown here for first time. Quantitative PCR showed that though both delta 12 omega 6 fatty acid desaturase genes FAD2-3 and FAD2-4 identified in cotton are induced under cold stress, FAD2-4 induction is significantly higher than FAD2-3. The induction of both isoforms was light regulated, in contrast a third isoform FAD2-2 was not affected by cold or light. Stress tolerance and light regulatory elements were identified in the predicted promoters of both FAD2-3 and FAD2-4 genes. Di-unsaturated fatty acid species rapidly increased in the microsomal fraction isolated from cotton leaves, following cold stress. Expression analysis patterns were correlated with the observed increase in both total and microsomal fatty acid unsaturation levels suggesting the direct role of the FAD2 genes in membrane adaptation to cold stress.
机译：脂质修饰酶在抗寒植物（例如谷物）的耐冷胁迫发展中起关键作用。但是，关于内源酶在诸如棉花之类的冷敏感物种中的作用知之甚少。在基因表达研究中使用了已知通过酰基链修饰参与适应低温的Delta 12脂肪酸去饱和酶（FAD2），以鉴定植物对低温的反应参数。此处首次显示了植物在冷胁迫下在mRNA水平上诱导的微粒体δ12脂肪酸去饱和酶的表达。定量PCR显示，尽管在寒冷胁迫下诱导了棉中鉴定的δ12ω6脂肪酸去饱和酶基因FAD2-3和FAD2-4，但FAD2-4的诱导显着高于FAD2-3。两种同工型的诱导均受光调节，相反，第三种同工型FAD2-2不受冷或光的影响。在FAD2-3和FAD2-4基因的预测启动子中鉴定了胁迫耐受性和光调节元件。冷胁迫后，从棉花叶中分离出的微粒体部分中的二不饱和脂肪酸种类迅速增加。表达分析模式与总脂肪酸和不饱和脂肪酸的不饱和水平增加相关，表明FAD2基因在膜适应冷胁迫中的直接作用。

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